Effects of glutathione on kinetics and structural properties of amphibian BbGSTP1-1

Effects of glutathione on the kinetics and structural properties of BbGSTP1-1 were investigated. The liganded state BbGSTP1-1 acquires the capacity to bind the hydrophobic molecules more avidly. Thus, GSH-binding produces significant conformational changes on BbGSTP1-1 which are transmitted to the h...

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Bibliographic Details
Published in:The international journal of biochemistry & cell biology 2003-04, Vol.35 (4), p.415-421
Main Authors: Pennelli, Alfonso, Sacchetta, Paolo, Catitti, Cesare, Amicarelli, Fernanda, Di Ilio, Carmine
Format: Article
Language:English
Subjects:
Amphibian
Animals
Binding Sites
Bufo bufo - embryology
Bufo bufo - metabolism
Carrier Proteins - metabolism
Embryo, Nonmammalian - enzymology
Flexibility
Glutathione
Glutathione - metabolism
Glutathione S-Transferase pi
Glutathione Transferase - metabolism
GST
Helminth Proteins
Isoenzymes - metabolism
Kinetics
Models, Molecular
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Summary:Effects of glutathione on the kinetics and structural properties of BbGSTP1-1 were investigated. The liganded state BbGSTP1-1 acquires the capacity to bind the hydrophobic molecules more avidly. Thus, GSH-binding produces significant conformational changes on BbGSTP1-1 which are transmitted to the hydrophobic binding site. Fluorescent experiments carried out with glutathione-analog S-methylglutathione suggest that the –SH group of tripeptide is essential for triggering protein conformational changes. It is argued that the capacity of BbGSTP1-1 to be modulated by GSH concentration allows it to play an efficient detoxication action in both aquatic and terrestrial environments.
ISSN:1357-2725
1878-5875
DOI:10.1016/S1357-2725(02)00253-4