Capillary zone electrophoresis of soil humic acid fractions obtained by coupling size-exclusion chromatography and polyacrylamide gel electrophoresis

Capillary zone electrophoresis (CZE) was used for characterisation of soil humic acid (HA) fractions obtained by coupling size-exclusion chromatography with polyacrylamide gel electrophoresis, on the basis of their molecular size and electrophoretic mobility. CZE was conducted using several low alka...

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Bibliographic Details
Published in:Journal of Chromatography A 2003-01, Vol.983 (1), p.263-270
Main Authors: Cavani, Luciano, Ciavatta, Claudio, Trubetskaya, Olga E, Reznikova, Olga I, Afanas’eva, Gaida V, Trubetskoj, Oleg A
Format: Article
Language:English
Subjects:
Chromatography, Gel - methods
Electrophoresis, Capillary - methods
Electrophoresis, Polyacrylamide Gel - methods
Humic acids
Humic Substances - analysis
Soil - analysis
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Summary:Capillary zone electrophoresis (CZE) was used for characterisation of soil humic acid (HA) fractions obtained by coupling size-exclusion chromatography with polyacrylamide gel electrophoresis, on the basis of their molecular size and electrophoretic mobility. CZE was conducted using several low alkaline buffers as background electrolyte (BGE): 50 m M carbonate, pH 9.0; 50 m M phosphate, pH 8.5; 50 m M borate, pH 8.3; 50 m M Tris–borate+1 m M EDTA+7 M urea+0.1% sodium dodecyl sulphate (SDS), pH 8.3. Independently of BGE conditions, the effective electrophoretic mobility of HA fractions were in good agreement with their molecular size. The better resolution of HA were obtained in Tris–borate–EDTA buffer with urea and SDS. This results indicated that CZE, mostly with BGE-contained disaggregating agents, is useful for separating HAs in fractions with different molecular sizes.
ISSN:0021-9673
DOI:10.1016/S0021-9673(02)01737-5