Effects of myo‐inositol on the in‐vitro maturation and subsequent development of mouse oocytes

BACKGROUND: The study aim was to assess whether the incorporation of myo‐inositol (MI) into culture medium could improve oocyte maturation in vitro. METHODS AND RESULTS: We performed a controlled prospective study using female ICR strain mice superovulated with pregnant mare’s serum gonadotrophins....

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Bibliographic Details
Published in:Human reproduction (Oxford) 2003-02, Vol.18 (2), p.408-416
Main Authors: Chiu, Tony Tak Yu, Rogers, Michael Scott, Briton‐Jones, Christine, Haines, Christopher
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Birth control
Calcium - metabolism
Cells, Cultured
Cellular Senescence - drug effects
Culture Media
Female
Fertilization in Vitro
Gynecology. Andrology. Obstetrics
Inositol - administration & dosage
Inositol - pharmacology
Intracellular Membranes - metabolism
Key words: embryo development/intracellular Ca2+ fluxes/mouse/myo‐inositol/oocyte maturation
Medical sciences
Meiosis - drug effects
Mice
Oocytes - drug effects
Oocytes - metabolism
Oocytes - physiology
Oscillometry
Ovarian Follicle - cytology
Sterility. Assisted procreation
Treatment Outcome
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Summary:BACKGROUND: The study aim was to assess whether the incorporation of myo‐inositol (MI) into culture medium could improve oocyte maturation in vitro. METHODS AND RESULTS: We performed a controlled prospective study using female ICR strain mice superovulated with pregnant mare’s serum gonadotrophins. Cumulus‐enclosed germinal vesicle (GV) oocytes were randomly cultured in medium with or without MI supplementation. The kinetics of GV breakdown after 4 h of incubation was significantly higher in oocytes incubated with 30 mmol/l of MI than in controls (P < 0.001). Accordingly, this concentration of MI was used for subsequent experiments. The proportion of metaphase II oocytes achieved after 24 h of culture, their fertilization and cleavage rates were significantly higher in the MI‐treated group (P < 0.01, P < 0.05, P < 0.05 respectively). This group also demonstrated significant improvement in postimplantation development after transferring the 2‐cell embryos to pseudopregnant mice. Confocal microscopy revealed spontaneous intracellular Ca2+ oscillations within competent GV oocytes and treatment with MI caused an earlier onset of these Ca2+ signals. CONCLUSIONS: Our results suggest that MI may affect meiotic progression of mouse GV oocytes possibly by enhancing the intracellular Ca2+ oscillations. Supplementation of MI in culture medium may be useful for human oocyte maturation.
ISSN:0268-1161
1460-2350
1460-2350
DOI:10.1093/humrep/deg113