Influence of Carboxymethyl Dextran and Ferric Citrate on the Adhesion of CHO Cells on Microcarriers

Due to the inherent risks of animal‐derived raw materials, the biopharmaceutical industry has an increasing demand for serum‐free and protein‐free media for industrial cell culture bioprocesses. The absence of serum often changes the characteristics of mammalian cells, especially growth, productivit...

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Bibliographic Details
Published in:Biotechnology progress 2003, Vol.19 (1), p.21-29
Main Authors: Landauer, Karlheinz, Wiederkum, Susanne, Dürrschmid, Markus, Klug, Helga, Simic, Gordana, Blüml, Gerald, Doblhoff-Dier, Otto
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Bioreactors - classification
Bioreactors - microbiology
Cell Adhesion - drug effects
Cell Adhesion - physiology
Cell Count
Cell Division - drug effects
Cell Division - physiology
CHO Cells - cytology
CHO Cells - drug effects
CHO Cells - physiology
Cricetinae
Culture Media - pharmacology
Dextrans - pharmacology
Dose-Response Relationship, Drug
Ferric Compounds - pharmacology
Fundamental and applied biological sciences. Psychology
Membranes, Artificial
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Summary:Due to the inherent risks of animal‐derived raw materials, the biopharmaceutical industry has an increasing demand for serum‐free and protein‐free media for industrial cell culture bioprocesses. The absence of serum often changes the characteristics of mammalian cells, especially growth, productivity, and adherence properties. This study is mainly focused on the influence of media additives on cell adherence characteristics. An array of different carboxymethyl dextrans and different ferric citrate concentrations was tested with a number of CHO clones, using standard cell culture Roux‐flasks and Cytoline 1 macroporous microcarriers. A prototype mixing system with controlled shear force input was developed as a screening system for adherence characteristics. The results of this evaluation revealed a negatively correlated dose‐dependent influence on adhesion for ferric citrate. It was also found that certain carboxymethyl dextrans are capable of increasing the adherence on Roux‐flasks and microcarriers.
ISSN:8756-7938
1520-6033
DOI:10.1021/bp025568l