Hepatitis C Virus NS2 Protein Inhibits Gene Expression from Different Cellular and Viral Promoters in Hepatic and Nonhepatic Cell Lines

The mechanisms leading to viral persistence and hepatocarcinogenesis in hepatitis C virus (HCV) infection are not fully understood. Recently, evidence has been accumulated that HCV viral proteins might interfere with gene expression of host cells. Here, we demonstrate that the amino-terminal portion...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2003-01, Vol.305 (2), p.260-266
Main Authors: Dumoulin, Franz Ludwig, von dem Bussche, Annette, Li, Jisu, Khamzina, Leila, Wands, Jack R., Sauerbruch, Tilman, Spengler, Ulrich
Format: Article
Language:English
Subjects:
Cell Line
Gene Expression
Hepatitis, Chronic - etiology
Humans
Promoter Regions, Genetic
Viral Nonstructural Proteins - physiology
Virus Replication
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Summary:The mechanisms leading to viral persistence and hepatocarcinogenesis in hepatitis C virus (HCV) infection are not fully understood. Recently, evidence has been accumulated that HCV viral proteins might interfere with gene expression of host cells. Here, we demonstrate that the amino-terminal portion of HCV NS2 protein inhibits the expression of reporter genes driven by different promoters or enhancer elements and also inhibits hepatitis B virus (HBV) gene expression and HBV DNA replication. The inhibitory effect of HCV NS2 on liver and non-liver-specific promoters and enhancer elements might be relevant for the pathogenesis of chronic HCV infection.
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.2002.1701