Amperometric Detection of Quantal Catecholamine Secretion from Individual Cells on Micromachined Silicon Chips

We have fabricated electrochemical electrodes in picoliter-sized wells for measuring catecholamine release from individual cells with millisecond resolution. Each well-electrode roughly conforms to the shape of the cell in order to capture a large fraction of released catecholamine with high time re...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2003-02, Vol.75 (3), p.518-524
Main Authors: Chen, Peng, Xu, Bai, Tokranova, Natalya, Feng, Xiaojun, Castracane, James, Gillis, Kevin D
Format: Article
Language:English
Subjects:
Analytical chemistry
Animals
Biological and medical sciences
Biosensors
Biotechnology
Catecholamines - analysis
Catecholamines - metabolism
Cattle
Cells
Chemistry
Chromaffin Cells - metabolism
Electrochemical methods
Electrochemistry - instrumentation
Electrochemistry - methods
Electrodes
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General, instrumentation
Integrated circuits
Methods. Procedures. Technologies
Miniaturization
Silicon
Various methods and equipments
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Summary:We have fabricated electrochemical electrodes in picoliter-sized wells for measuring catecholamine release from individual cells with millisecond resolution. Each well-electrode roughly conforms to the shape of the cell in order to capture a large fraction of released catecholamine with high time resolution. Using this device, we can resolve spikes in amperometric current corresponding to quantal catecholamine release via exocytosis. In addition, we have combined amperometric recording on the chip with patch-clamp recordings of membrane capacitance as an assay of exocytosis. A quantitative comparison of the two methods suggests that a large fraction of catecholamine release is oxidized on the surface of the well-electrode. This technology has applications in cell-based biosensor development, high-throughput screening of drugs, and basic science investigations.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac025802m