Global organellar proteomics

Cataloging the proteomes of single-celled microorganisms, cells, biological fluids, tissue and whole organisms is being undertaken at a rapid pace as advances are made in protein and peptide separation, detection and identification. For metazoans, subcellular organelles represent attractive targets...

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Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 2003-02, Vol.21 (2), p.82-88
Main Authors: Taylor, Steven W, Fahy, Eoin, Ghosh, Soumitra S
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Biological and medical sciences
Biotechnology
Cell Nucleus - chemistry
Cell Nucleus - genetics
Cell Nucleus - metabolism
Chloroplasts - chemistry
Chloroplasts - genetics
Chloroplasts - metabolism
Diverse techniques
Endoplasmic Reticulum - chemistry
Endoplasmic Reticulum - genetics
Endoplasmic Reticulum - metabolism
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling - methods
Gene Expression Regulation
Genes
Golgi Apparatus - chemistry
Golgi Apparatus - genetics
Golgi Apparatus - metabolism
Lysosomes - chemistry
Lysosomes - genetics
Lysosomes - metabolism
Microorganisms
Mitochondria - genetics
Mitochondria - metabolism
Mitochondrial DNA
Molecular and cellular biology
Molecular Sequence Data
Organelles - genetics
Organelles - metabolism
Peroxisomes - chemistry
Peroxisomes - genetics
Peroxisomes - metabolism
Phagosomes - chemistry
Phagosomes - genetics
Phagosomes - metabolism
Proteins - chemistry
Proteins - genetics
Proteins - metabolism
Proteomics
Proteomics - methods
Species Specificity
Studies
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Summary:Cataloging the proteomes of single-celled microorganisms, cells, biological fluids, tissue and whole organisms is being undertaken at a rapid pace as advances are made in protein and peptide separation, detection and identification. For metazoans, subcellular organelles represent attractive targets for global proteome analysis because they represent discrete functional units, their complexity in protein composition is reduced relative to whole cells and, when abundant cytoskeletal proteins are removed, lower abundance proteins specific to the organelle are revealed. Here, we review recent literature on the global analysis of subcellular organelles and briefly discuss how that information is being used to elucidate basic biological processes that range from cellular signaling pathways through protein–protein interactions to differential expression of proteins in response to external stimuli. We assess the relative merits of the different methods used and discuss issues and future directions in the field.
ISSN:0167-7799
1879-3096
DOI:10.1016/S0167-7799(02)00037-9