Expression of Cytosolic and Plastid Acetyl-Coenzyme A Carboxylase Genes in Young Wheat Plants

• Published in: Plant physiology (Bethesda) 2003-02, Vol.131 (2), p.763-772
• Main Authors: Podkowinski, Jan, Jelenska, Joanna, Sirikhachornkit, Anchalee, Ellen Zuther, Haselkorn, Robert, Gornicki, Piotr
• Format: Article
• Language: English
• Subjects: Acetyl-CoA Carboxylase - genetics; Acetyl-CoA Carboxylase - metabolism; Agronomy. Soil science and plant productions; Biochemical Processes and Macromolecular Structures; Biological and medical sciences; Blotting, Northern; Chloroplasts; Classical and quantitative genetics. Population genetics. Molecular genetics; Complementary DNA; Cytosol - enzymology; Economic plant physiology; Fatty acids; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Generalities. Genetics. Plant material; Genes; Genetics and breeding of economic plants; Introns; Leaves; Messenger RNA; Metabolism; Molecular genetics; Multigene Family; Net assimilation, photosynthesis, carbon metabolism. Photorespiration, respiration, fermentation (anoxia, hypoxia); Nutrition. Photosynthesis. Respiration. Metabolism; Phosphoglycerate Kinase - metabolism; Photosynthesis, respiration. Anabolism, catabolism; Plant Leaves - enzymology; Plant Leaves - genetics; Plant physiology and development; Plants; Plastids; Plastids - enzymology; Polyploidy; Reverse Transcriptase Polymerase Chain Reaction; RNA; RNA, Messenger - genetics; RNA, Messenger - metabolism; Roots; Triticum - enzymology; Triticum - genetics; Triticum - growth & development; Wheat
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.013169

Expression of cytosolic and plastid acetyl-coenzyme A carboxylase (ACCase) gene families at the mRNA level was analyzed in developing wheat (Triticum aestivum) plants. The major plastid ACCase mRNA level is high in the middle part of the plant and low in roots and leaf blades. An alternative plastid ACCase transcript initiated at a different promoter and using an alternative 5′ splice site for the first intron accumulates to its highest level in roots. Cytosolic ACCase mRNA also consists of two species, one of which is present at approximately a constant level, whereas the other accumulates to a high level in the lower sheath section. It is likely that different promoters are also responsible for the two forms of cytosolic ACCase mRNA. The abundances of cytosolic and plastid ACCase mRNAs in the sheath section of the plant are similar. ACCase protein level is significantly lower in the leaf blades, in parallel with changes in the total ACCase mRNA level. Homoeologous ACCase genes show the same expression patterns and similar mRNA levels, suggesting that none of the genes was silenced or acquired new tissue specificity after polyploidization.