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A monoclonal-based IgM capture ELISA for detection of antibodies to 22 and 41 kDa membrane antigens of Toxoplasma gondii
A murine monoclonal antibody which reacts to 22 and 41 kDa Toxoplasma gondii surface antigens was employed in an IgM capture enzyme-linked immunosorbent assay (ELISA). A total of 125 patients' sera were tested in the monoclonal-based assay. When compared with a commercial ELISA test (Abbott Tox...
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| Published in: | Parasitology 1992-02, Vol.104 (1), p.25-31 |
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| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c457t-13c2866771b4b931ec1bf134f0781f6250a04097c11f0b3183e0f8cccbfc7f173 |
| container_end_page | 31 |
| container_issue | 1 |
| container_start_page | 25 |
| container_title | Parasitology |
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| creator | Wee, J. L. K. Ho, L. C. Yap, E. H. Singh, M. |
| description | A murine monoclonal antibody which reacts to 22 and 41 kDa Toxoplasma gondii surface antigens was employed in an IgM capture enzyme-linked immunosorbent assay (ELISA). A total of 125 patients' sera were tested in the monoclonal-based assay. When compared with a commercial ELISA test (Abbott Toxo-M EIA) which uses polyclonal anti-T. gondii antibodies, good correlation (Pearsons coefficient r = 0.91) was observed. The specificity of the assay was studied by testing a panel of control sera obtained from healthy individuals and blood transfusion donors; all sera gave negative results. Serum samples positive for T. gondii antibodies were treated with 2-mercaptoethanol (2-ME) to demonstrate the specificity of the test for IgM antibodies. Reactivity of these sera was lost after the treatment. The test is not subject to interference by rheumatoid factor as sera positive for rheumatoid factor were negative in the assay. Reproducibility was good with the coefficients of variation for within-day tests below 10% and not exceeding 18% for day-to-day tests. The monoclonal-based assay is simple to perform and appears to be a viable test for diagnosis of T. gondii infection. |
| doi_str_mv | 10.1017/S0031182000060765 |
| format | article |
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L. K. ; Ho, L. C. ; Yap, E. H. ; Singh, M.</creator><creatorcontrib>Wee, J. L. K. ; Ho, L. C. ; Yap, E. H. ; Singh, M.</creatorcontrib><description>A murine monoclonal antibody which reacts to 22 and 41 kDa Toxoplasma gondii surface antigens was employed in an IgM capture enzyme-linked immunosorbent assay (ELISA). A total of 125 patients' sera were tested in the monoclonal-based assay. When compared with a commercial ELISA test (Abbott Toxo-M EIA) which uses polyclonal anti-T. gondii antibodies, good correlation (Pearsons coefficient r = 0.91) was observed. The specificity of the assay was studied by testing a panel of control sera obtained from healthy individuals and blood transfusion donors; all sera gave negative results. Serum samples positive for T. gondii antibodies were treated with 2-mercaptoethanol (2-ME) to demonstrate the specificity of the test for IgM antibodies. Reactivity of these sera was lost after the treatment. The test is not subject to interference by rheumatoid factor as sera positive for rheumatoid factor were negative in the assay. Reproducibility was good with the coefficients of variation for within-day tests below 10% and not exceeding 18% for day-to-day tests. The monoclonal-based assay is simple to perform and appears to be a viable test for diagnosis of T. gondii infection.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182000060765</identifier><identifier>PMID: 1614737</identifier><identifier>CODEN: PARAAE</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animals ; Antibodies, Monoclonal - immunology ; Antibodies, Protozoan - blood ; ANTICORPS MONOCLONAL ; ANTICUERPOS MONOCLONALES ; ANTIGENE ; ANTIGENOS ; ANTIGENS ; Antigens, Protozoan - immunology ; Biological and medical sciences ; Blood Donors ; DIAGNOSIS ; DIAGNOSTIC ; DIAGNOSTIC VALUE ; DIAGNOSTICO ; ELISA ; Enzyme-Linked Immunosorbent Assay ; Humans ; IgM ELISA ; Immunoblotting ; Immunoglobulin G - immunology ; Immunoglobulin M - blood ; IMMUNOGLOBULINE ; IMMUNOGLOBULINS ; Immunological methods for diagnosis and exploration ; Immunopathology ; INMUNOGLOBULINA ; Medical sciences ; MONOCLONAL ANTIBODIES ; monoclonal antibody ; Reproducibility of Results ; Sensitivity and Specificity ; Serological tests ; TEST ELISA ; TOXOPLASMA ; Toxoplasma - immunology ; Toxoplasma gondii</subject><ispartof>Parasitology, 1992-02, Vol.104 (1), p.25-31</ispartof><rights>Copyright © Cambridge University Press 1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-13c2866771b4b931ec1bf134f0781f6250a04097c11f0b3183e0f8cccbfc7f173</citedby><cites>FETCH-LOGICAL-c457t-13c2866771b4b931ec1bf134f0781f6250a04097c11f0b3183e0f8cccbfc7f173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182000060765/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,55689</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5286694$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1614737$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wee, J. L. K.</creatorcontrib><creatorcontrib>Ho, L. C.</creatorcontrib><creatorcontrib>Yap, E. H.</creatorcontrib><creatorcontrib>Singh, M.</creatorcontrib><title>A monoclonal-based IgM capture ELISA for detection of antibodies to 22 and 41 kDa membrane antigens of Toxoplasma gondii</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>A murine monoclonal antibody which reacts to 22 and 41 kDa Toxoplasma gondii surface antigens was employed in an IgM capture enzyme-linked immunosorbent assay (ELISA). A total of 125 patients' sera were tested in the monoclonal-based assay. When compared with a commercial ELISA test (Abbott Toxo-M EIA) which uses polyclonal anti-T. gondii antibodies, good correlation (Pearsons coefficient r = 0.91) was observed. The specificity of the assay was studied by testing a panel of control sera obtained from healthy individuals and blood transfusion donors; all sera gave negative results. Serum samples positive for T. gondii antibodies were treated with 2-mercaptoethanol (2-ME) to demonstrate the specificity of the test for IgM antibodies. Reactivity of these sera was lost after the treatment. The test is not subject to interference by rheumatoid factor as sera positive for rheumatoid factor were negative in the assay. Reproducibility was good with the coefficients of variation for within-day tests below 10% and not exceeding 18% for day-to-day tests. The monoclonal-based assay is simple to perform and appears to be a viable test for diagnosis of T. gondii infection.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Protozoan - blood</subject><subject>ANTICORPS MONOCLONAL</subject><subject>ANTICUERPOS MONOCLONALES</subject><subject>ANTIGENE</subject><subject>ANTIGENOS</subject><subject>ANTIGENS</subject><subject>Antigens, Protozoan - immunology</subject><subject>Biological and medical sciences</subject><subject>Blood Donors</subject><subject>DIAGNOSIS</subject><subject>DIAGNOSTIC</subject><subject>DIAGNOSTIC VALUE</subject><subject>DIAGNOSTICO</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Humans</subject><subject>IgM ELISA</subject><subject>Immunoblotting</subject><subject>Immunoglobulin G - immunology</subject><subject>Immunoglobulin M - blood</subject><subject>IMMUNOGLOBULINE</subject><subject>IMMUNOGLOBULINS</subject><subject>Immunological methods for diagnosis and exploration</subject><subject>Immunopathology</subject><subject>INMUNOGLOBULINA</subject><subject>Medical sciences</subject><subject>MONOCLONAL ANTIBODIES</subject><subject>monoclonal antibody</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Serological tests</subject><subject>TEST ELISA</subject><subject>TOXOPLASMA</subject><subject>Toxoplasma - immunology</subject><subject>Toxoplasma gondii</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNqFkl-P1CAUxYnRrOPqB9DEhAfjW5VbWmgfx_3nJGOMO7vxkQCFht22zEKbjN9eup2sDybKCwnndw_ce0DoLZBPQIB_3hFCAaqcpMUIZ-UztIKC1VkFDJ6j1Sxns_4SvYrxbqYoy0_QSZILTvkKHda494PXnR9klykZTYM37Tes5X6cgsEX281uja0PuDGj0aPzA_YWy2F0yjfORDx6nOfpoMEF4PtziXvTqyAH8wi1ZohzwY0_-H0nYy9x64fGudfohZVdNG-O-ym6vby4Ofuabb9fbc7W20wXJR8zoDqvGOMcVKFqCkaDskALS3gFluUlkaQgNdcAligKFTXEVlprZTW3wOkp-rj47oN_mEwcRe-iNl2XXuinKDglOUsG_wWBlbQueJ5AWEAdfIzBWLEPrpfhlwAi5ljEX7GkmvdH80n1pvlTseSQ9A9HXUYtO5vmp118wsp5BnWRsGzBXBzN4UmW4V6wZFMKdvVDfPl5uT2_zmtxnfh3C2-lF7INyfJ2V1NCysc76bEPmQJzTWvEnZ9C-gfxH538BsPRuKU</recordid><startdate>19920201</startdate><enddate>19920201</enddate><creator>Wee, J. L. K.</creator><creator>Ho, L. C.</creator><creator>Yap, E. H.</creator><creator>Singh, M.</creator><general>Cambridge University Press</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19920201</creationdate><title>A monoclonal-based IgM capture ELISA for detection of antibodies to 22 and 41 kDa membrane antigens of Toxoplasma gondii</title><author>Wee, J. L. K. ; Ho, L. C. ; Yap, E. H. ; Singh, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-13c2866771b4b931ec1bf134f0781f6250a04097c11f0b3183e0f8cccbfc7f173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibodies, Protozoan - blood</topic><topic>ANTICORPS MONOCLONAL</topic><topic>ANTICUERPOS MONOCLONALES</topic><topic>ANTIGENE</topic><topic>ANTIGENOS</topic><topic>ANTIGENS</topic><topic>Antigens, Protozoan - immunology</topic><topic>Biological and medical sciences</topic><topic>Blood Donors</topic><topic>DIAGNOSIS</topic><topic>DIAGNOSTIC</topic><topic>DIAGNOSTIC VALUE</topic><topic>DIAGNOSTICO</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Humans</topic><topic>IgM ELISA</topic><topic>Immunoblotting</topic><topic>Immunoglobulin G - immunology</topic><topic>Immunoglobulin M - blood</topic><topic>IMMUNOGLOBULINE</topic><topic>IMMUNOGLOBULINS</topic><topic>Immunological methods for diagnosis and exploration</topic><topic>Immunopathology</topic><topic>INMUNOGLOBULINA</topic><topic>Medical sciences</topic><topic>MONOCLONAL ANTIBODIES</topic><topic>monoclonal antibody</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Serological tests</topic><topic>TEST ELISA</topic><topic>TOXOPLASMA</topic><topic>Toxoplasma - immunology</topic><topic>Toxoplasma gondii</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wee, J. L. K.</creatorcontrib><creatorcontrib>Ho, L. C.</creatorcontrib><creatorcontrib>Yap, E. H.</creatorcontrib><creatorcontrib>Singh, M.</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wee, J. L. K.</au><au>Ho, L. C.</au><au>Yap, E. H.</au><au>Singh, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A monoclonal-based IgM capture ELISA for detection of antibodies to 22 and 41 kDa membrane antigens of Toxoplasma gondii</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>1992-02-01</date><risdate>1992</risdate><volume>104</volume><issue>1</issue><spage>25</spage><epage>31</epage><pages>25-31</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><coden>PARAAE</coden><abstract>A murine monoclonal antibody which reacts to 22 and 41 kDa Toxoplasma gondii surface antigens was employed in an IgM capture enzyme-linked immunosorbent assay (ELISA). A total of 125 patients' sera were tested in the monoclonal-based assay. When compared with a commercial ELISA test (Abbott Toxo-M EIA) which uses polyclonal anti-T. gondii antibodies, good correlation (Pearsons coefficient r = 0.91) was observed. The specificity of the assay was studied by testing a panel of control sera obtained from healthy individuals and blood transfusion donors; all sera gave negative results. Serum samples positive for T. gondii antibodies were treated with 2-mercaptoethanol (2-ME) to demonstrate the specificity of the test for IgM antibodies. Reactivity of these sera was lost after the treatment. The test is not subject to interference by rheumatoid factor as sera positive for rheumatoid factor were negative in the assay. Reproducibility was good with the coefficients of variation for within-day tests below 10% and not exceeding 18% for day-to-day tests. The monoclonal-based assay is simple to perform and appears to be a viable test for diagnosis of T. gondii infection.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>1614737</pmid><doi>10.1017/S0031182000060765</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0031-1820 |
| ispartof | Parasitology, 1992-02, Vol.104 (1), p.25-31 |
| issn | 0031-1820 1469-8161 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73026097 |
| source | Cambridge University Press:JISC Collections:Full Collection Digital Archives (STM and HSS) (218 titles) |
| subjects | Animals Antibodies, Monoclonal - immunology Antibodies, Protozoan - blood ANTICORPS MONOCLONAL ANTICUERPOS MONOCLONALES ANTIGENE ANTIGENOS ANTIGENS Antigens, Protozoan - immunology Biological and medical sciences Blood Donors DIAGNOSIS DIAGNOSTIC DIAGNOSTIC VALUE DIAGNOSTICO ELISA Enzyme-Linked Immunosorbent Assay Humans IgM ELISA Immunoblotting Immunoglobulin G - immunology Immunoglobulin M - blood IMMUNOGLOBULINE IMMUNOGLOBULINS Immunological methods for diagnosis and exploration Immunopathology INMUNOGLOBULINA Medical sciences MONOCLONAL ANTIBODIES monoclonal antibody Reproducibility of Results Sensitivity and Specificity Serological tests TEST ELISA TOXOPLASMA Toxoplasma - immunology Toxoplasma gondii |
| title | A monoclonal-based IgM capture ELISA for detection of antibodies to 22 and 41 kDa membrane antigens of Toxoplasma gondii |
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