Clinically relevant thermal preconditioning attenuates ischemia-reperfusion injury

• Published in: The Journal of surgical research 2003, Vol.109 (1), p.24-30
• Main Authors: McCormick, P.H, Chen, G, Tlerney, S, Kelly, C.J, Bouchier-Hayes, D.J
• Format: Article
• Language: English
• Subjects: Animals; Aorta; Biological and medical sciences; Blotting, Western; Body Temperature; Bronchoalveolar Lavage Fluid - chemistry; Capillary Permeability; clinically relevant; Constriction; Edema; heat shock protein 72; Heat-Shock Proteins - analysis; Hot Temperature; HSP72 Heat-Shock Proteins; ischemia-reperfusion; Ischemic Preconditioning; Lung - blood supply; Lung - chemistry; Lung - pathology; Male; Medical sciences; Miscellaneous; Organ Size; Peroxidase - analysis; Pneumology; Proteins - analysis; Rats; Rats, Sprague-Dawley; Reperfusion Injury - prevention & control; Respiratory system : syndromes and miscellaneous diseases; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; thermal preconditioning
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1016/S0022-4804(02)00035-5

Introduction. Thermal preconditioning has previously been shown to attenuate ischemia-reperfusion induced injuries, possible due to increased expression of heat shock proteins (HSP). The model of thermal preconditioning used, however, was not clinically relevant as preconditioning was to 41°C, leading to cellular damage. Our aim was thus to establish a novel and clinically applicable method of preconditioning. Materials and Methods. Twenty-six male Sprague-Dawley rats were split into three groups (nine control, nine ischemia-reperfusion, and eight preconditioned followed by ischemia-reperfusion). To precondition the animals, they were anesthetized and, using a water bath, their core temperature was raised by 1°C for 15 min once a day for five successive days. I/R injury consisted of 30 min of aortic cross-clamping followed by 120 min of reperfusion; control animals had a laparotomy only. Indicators of lung injury were tissue myeloperoxidase, broncho-alveolar lavage protein concentration, and tissue edema. Tissue heat shock protein expression was detected by Western blot analysis. Results. Lower torso ischemia-reperfusion causes significant lung injury versus control, with raised levels of myeloperoxidase 4.53 iu/g to 7.88 iu/g ( P < 0.05), raised B.A.L. protein concentration 419 μg/ml to 684 μg/ml ( P < 0.05) and altered wet dry ratio 4.63 to 5.50. Clinically relevant thermal preconditioning attenuates all of these parameters back to control levels: myeloperoxidase 3.87 iu/g ( P < 0.05 vs I/R), B.A.L. to 284 μg/ml ( P < 0.01 vs I/R) and wet dry ratio to 4.44 ( P < 0.05 vs I/R). Western blot demonstrated increased expression of H.S.P. 72 in the preconditioned group versus control and I/R alone. Western blot demonstrated increased expression of HSP72 in the preconditioned group vs control and I/R alone. Conclusions. We conclude that clinically applicable thermal preconditioning can attenuate ischemia-reperfusion induced lung injury, possibly through increased expression of HSP72.