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In vitro culture of Glugea sp
Recently the low host specificity of some microsporidians has been demonstrated and it has been indicated that many of these micro-organisms could be transmitted from invertebrates to mammals and adapt to changes in temperature. In this work, we demonstrate the first successful in vitro culture of a...
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| Published in: | Veterinary parasitology 2003-03, Vol.112 (3), p.185-196 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c361t-b1d158f515516393857d5ed3f8a5a81edbf88beb6e3ca8ee11ac62bcfbce61003 |
| container_end_page | 196 |
| container_issue | 3 |
| container_start_page | 185 |
| container_title | Veterinary parasitology |
| container_volume | 112 |
| creator | Lores, B. Rosales, M.J. Mascaró, C. Osuna, A. |
| description | Recently the low host specificity of some microsporidians has been demonstrated and it has been indicated that many of these micro-organisms could be transmitted from invertebrates to mammals and adapt to changes in temperature. In this work, we demonstrate the first successful in vitro culture of a fish microsporidia of the genus
Glugea on larval cells of the mosquito
Aedes albopictus at 28
°C, and we show ultrastructural aspects of the different life cycle stages. It was impossible on salmon cells CHSE-214 at 21
°C. This study will be valuable for further work in biochemistry and immunology in addition to chemotherapy for microsporidiosis humans and animals. |
| doi_str_mv | 10.1016/S0304-4017(02)00411-9 |
| format | article |
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Glugea on larval cells of the mosquito
Aedes albopictus at 28
°C, and we show ultrastructural aspects of the different life cycle stages. It was impossible on salmon cells CHSE-214 at 21
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Glugea on larval cells of the mosquito
Aedes albopictus at 28
°C, and we show ultrastructural aspects of the different life cycle stages. It was impossible on salmon cells CHSE-214 at 21
°C. This study will be valuable for further work in biochemistry and immunology in addition to chemotherapy for microsporidiosis humans and animals.</description><subject>Aedes - cytology</subject><subject>Aedes - parasitology</subject><subject>Aedes albopictus cells</subject><subject>Animals</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Line</subject><subject>CHSE-214 cells</subject><subject>Culture</subject><subject>Fishes - parasitology</subject><subject>Glugea</subject><subject>Larva - cytology</subject><subject>Larva - parasitology</subject><subject>Life Cycle Stages - physiology</subject><subject>Microgemma</subject><subject>Microsporidia - cytology</subject><subject>Microsporidia - physiology</subject><subject>Microsporidia - ultrastructure</subject><subject>Salmon - parasitology</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqFkDFPwzAQhS0EoqXwE4oyIRgCd3GcOBNCFZRKlRiA2XLsCzJKm2Inlfj3pE0FI9Mt37un9zE2RbhFwOzuFTikcQqYX0NyA5AixsURG6PMeZwIAcds_IuM2FkIn9BTkOWnbISJKBCLdMymi3W0da1vItPVbecpaqpoXncfpKOwOWcnla4DXRzuhL0_Pb7NnuPly3wxe1jGhmfYxiVaFLISKARmvOBS5FaQ5ZXUQkskW1ZSllRmxI2WRIjaZElpqtJQhgB8wq6GvxvffHUUWrVywVBd6zU1XVA5B84LKXpQDKDxTQieKrXxbqX9t0JQOy9q70XtRitI1N6LKvrc5aGgK1dk_1IHET1wPwDUz9w68ioYR2tD1nkyrbKN-6fiBxkhcGc</recordid><startdate>20030310</startdate><enddate>20030310</enddate><creator>Lores, B.</creator><creator>Rosales, M.J.</creator><creator>Mascaró, C.</creator><creator>Osuna, A.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030310</creationdate><title>In vitro culture of Glugea sp</title><author>Lores, B. ; Rosales, M.J. ; Mascaró, C. ; Osuna, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-b1d158f515516393857d5ed3f8a5a81edbf88beb6e3ca8ee11ac62bcfbce61003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Aedes - cytology</topic><topic>Aedes - parasitology</topic><topic>Aedes albopictus cells</topic><topic>Animals</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Line</topic><topic>CHSE-214 cells</topic><topic>Culture</topic><topic>Fishes - parasitology</topic><topic>Glugea</topic><topic>Larva - cytology</topic><topic>Larva - parasitology</topic><topic>Life Cycle Stages - physiology</topic><topic>Microgemma</topic><topic>Microsporidia - cytology</topic><topic>Microsporidia - physiology</topic><topic>Microsporidia - ultrastructure</topic><topic>Salmon - parasitology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lores, B.</creatorcontrib><creatorcontrib>Rosales, M.J.</creatorcontrib><creatorcontrib>Mascaró, C.</creatorcontrib><creatorcontrib>Osuna, A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lores, B.</au><au>Rosales, M.J.</au><au>Mascaró, C.</au><au>Osuna, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro culture of Glugea sp</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2003-03-10</date><risdate>2003</risdate><volume>112</volume><issue>3</issue><spage>185</spage><epage>196</epage><pages>185-196</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>Recently the low host specificity of some microsporidians has been demonstrated and it has been indicated that many of these micro-organisms could be transmitted from invertebrates to mammals and adapt to changes in temperature. In this work, we demonstrate the first successful in vitro culture of a fish microsporidia of the genus
Glugea on larval cells of the mosquito
Aedes albopictus at 28
°C, and we show ultrastructural aspects of the different life cycle stages. It was impossible on salmon cells CHSE-214 at 21
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| fulltext | fulltext |
| identifier | ISSN: 0304-4017 |
| ispartof | Veterinary parasitology, 2003-03, Vol.112 (3), p.185-196 |
| issn | 0304-4017 1873-2550 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73033985 |
| source | Elsevier |
| subjects | Aedes - cytology Aedes - parasitology Aedes albopictus cells Animals Cell Culture Techniques - methods Cell Line CHSE-214 cells Culture Fishes - parasitology Glugea Larva - cytology Larva - parasitology Life Cycle Stages - physiology Microgemma Microsporidia - cytology Microsporidia - physiology Microsporidia - ultrastructure Salmon - parasitology |
| title | In vitro culture of Glugea sp |
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