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Integration of common bean (Phaseolus vulgaris L.) linkage and chromosomal maps

Fluorescent in situ hybridisation of pooled, closely linked RFLP markers was used to integrate the genetic linkage map and the mitotic chromosome map of the common bean. Pooled RFLP probes showed clear and reproducible signals and allowed the assignment of all linkage groups to the chromosomes of tw...

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Published in:Theoretical and applied genetics 2003, Vol.106 (2), p.205-212
Main Authors: PEDROSA, A, VALLEJOS, C. E, BACHMAIR, A, SCHWEIZER, D
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VALLEJOS, C. E
BACHMAIR, A
SCHWEIZER, D
description Fluorescent in situ hybridisation of pooled, closely linked RFLP markers was used to integrate the genetic linkage map and the mitotic chromosome map of the common bean. Pooled RFLP probes showed clear and reproducible signals and allowed the assignment of all linkage groups to the chromosomes of two Phaseolus vulgaris cultivars, Saxa and Calima. Low extension values for signals originating from clustered RFLPs suggest that these clones are physically close to each other and that clusters in the genetic map are not a result of suppression of recombination due to the occurrence of chromosome rearrangements. For linkage group K, clustering of markers could be associated with proximity to centromeres. High variation in the number of 45S rDNA loci was observed among cultivars, suggesting that these terminal sites are highly recombinogenic in common bean.
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source Springer Nature
subjects Biological and medical sciences
Chromosomes
Classical genetics, quantitative genetics, hybrids
Cloning
Cultivars
DNA, Ribosomal - genetics
Fabaceae - genetics
Fundamental and applied biological sciences. Psychology
Genetic Linkage
Genetics of eukaryotes. Biological and molecular evolution
In Situ Hybridization, Fluorescence
Physical Chromosome Mapping
Polymorphism, Restriction Fragment Length
Pteridophyta, spermatophyta
Seeds
Vegetals
title Integration of common bean (Phaseolus vulgaris L.) linkage and chromosomal maps
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