Characterization of diacetylenic liposomes as carriers for oral vaccines

In order to evaluate liposomes as vehicle for oral vaccines the characterization and stability of polymerized and non-polymerized liposomes were examined. Mixtures of 1,2-bis(10,12-tricosadiynoyl)- sn-glycero-3 phosphocholine) (DC8,9PC) with saturated 1,2-dimiristoyl- sn-glycero-3-phosphocholine in...

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Bibliographic Details
Published in:Chemistry and physics of lipids 2003, Vol.122 (1), p.191-203
Main Authors: Alonso-Romanowski, Silvia, Chiaramoni, Nadia S, Lioy, Virginia S, Gargini, Ricardo A, Viera, Liliana I, Taira, Maria C
Format: Article
Language:English
Subjects:
Acetylene - chemistry
Administration, Oral
Calorimetry, Differential Scanning
Diacetylenic lipids
Drug Carriers
EVs
Liposomes
Membrane Lipids - chemistry
Microscopy, Electron
Oral delivery
Polymerization
Stability digestive tract
Vaccines - administration & dosage
Vaccines - chemistry
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Summary:In order to evaluate liposomes as vehicle for oral vaccines the characterization and stability of polymerized and non-polymerized liposomes were examined. Mixtures of 1,2-bis(10,12-tricosadiynoyl)- sn-glycero-3 phosphocholine) (DC8,9PC) with saturated 1,2-dimiristoyl- sn-glycero-3-phosphocholine in molar ratio 1:1 were used. Saturated and non-saturated lipids were combined to give a chemically modified membrane by UV polymerization derived from DC8,9PC. Characterization was carried out by electronic microscopy, differential scanning calorimetry (DSC) and by hydrophobicity factor (HF). The stability towards the digestive tract (including saliva): acidic solutions, bile and pancreatin are compared to buffer pH 7.4, measuring the release of Glucose-6-phosphate or bovine plasma albumin entrapment. The polymerized liposomes showed further augmentation of the HF and the size. DSC showed phase separation and lower Tt if compared to data obtained for DC8,9PC. The HF, as main factor is discussed in relation to in vitro stability, suggesting that polymerized and non-polymerized liposomes would serve effectively as an oral delivery vehicle.
ISSN:0009-3084
1873-2941
DOI:10.1016/S0009-3084(02)00190-1