Biochemical changes in arthritic rats: dehydroascorbic and ascorbic acid levels

The objective of this work was to evaluate the use of vitamin C as a biomarker in the inflammatory phase of the rat adjuvant arthritis and to correlate it with other parameters used for disease evaluation. Paw swelling was used for physical evaluation and the levels of ascorbate and dehydroascorbate...

Full description

Saved in:
Bibliographic Details
Published in:European journal of pharmaceutical sciences 2003-02, Vol.18 (2), p.185-189
Main Authors: Simões, Sandra I.D, Eleutério, Carla V, Cruz, M.Eugénia M, Corvo, M.Luı́sa, Martins, M.Bárbara F
Format: Article
Language:English
Subjects:
Adjuvant arthritis
Analysis
Animals
Arthritis, Experimental - blood
Ascorbic acid
Ascorbic Acid - blood
Ascorbic Acid - chemistry
Biological and medical sciences
Dehydroascorbic acid
Dehydroascorbic Acid - blood
Dehydroascorbic Acid - chemistry
General pharmacology
High-performance liquid chromatography
Inflammatory biomarkers
Male
Medical sciences
Pharmacology. Drug treatments
Rats
Rats, Wistar
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The objective of this work was to evaluate the use of vitamin C as a biomarker in the inflammatory phase of the rat adjuvant arthritis and to correlate it with other parameters used for disease evaluation. Paw swelling was used for physical evaluation and the levels of ascorbate and dehydroascorbate in the serum of male rats, before and after adjuvant arthritis induction, were quantified by a high-performance liquid chromatography method (HPLC). The optimised HPLC assay enabled the quantification of both forms of the vitamin in rat sera, with the same extraction method and using different detectors, instead of obtaining dehydroascorbate by subtraction of the total ascorbate measurement. This method was used to follow the severity of adjuvant arthritis and the results were correlated with other already established disease activity parameters. A decrease of ascorbic acid and dehydroascorbic acid was observed with the increase of right paw circumference during the course of adjuvant arthritis. The disease associated changes in the serum concentrations of ascorbic acid, from biosynthesis and from recycling, can be evaluated by the direct quantification of dehydroascorbic acid. This provides some evidence for the potential of the quantification of these biomarkers to study the disease activity, and as a tool for the establishment of therapeutic protocols, to evaluate the anti-inflammatory effect of new drugs or formulations.
ISSN:0928-0987
1879-0720
DOI:10.1016/S0928-0987(02)00258-0