Extracellular 2-chloroadenosine and ATP stimulate volume-sensitive Cl − current and calcium mobilization in human tracheal 9HTEo− cells

• Published in: FEBS letters 1992-06, Vol.304 (1), p.61-65
• Main Authors: Galietta, Luis J.V., Rasola, Andrea, Rugolo, Michela, Zottini, Michela, Mastrocola, Teresa, Gruenert, Dieter C., Romeo, Giovanni
• Format: Article
• Language: English
• Subjects: 2-CADO; 2-Chloroadenosine; 2-Chloroadenosine - pharmacology; 3,7-dimethyl-1-propargylxanthine; Adenosine Triphosphate - pharmacology; Airway epithelium; ATP; Biological and medical sciences; Calcium - metabolism; Cell Line; Cell physiology; Cell volume; Chloride Channels; Chlorides - metabolism; Cl − current; Cl− current; DMPX; EGTA; ethyleneglycol-bis-(β-aminoethyl)-N,N,N′,N′-tetraacetic acid; Fundamental and applied biological sciences. Psychology; Fura-2; HEPES; Humans; Intracellular Ca 2; Intracellular Ca2; Ion Channels - metabolism; Membrane and intracellular transports; Membrane Potentials; Membrane Proteins - metabolism; Molecular and cellular biology; N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid; Theobromine - analogs & derivatives; Theobromine - pharmacology; Trachea - cytology; Trachea - drug effects; Trachea - metabolism
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(92)80589-9

The perforated-patch whole-cell technique was used to record membrane currents in epithelial cells (9HTEo−) obtained from the human tracheal epithelium. Extracellular application of 2-chloroadenosine and ATP (0.01–100 μM) caused activation of Cl − currents similar to those regulated by cell volume in airway and intestinal cells. This response was inhibited by increasing extracellular osmolality, by omission of extracellular Ca 2+ or by the addition of the A 2 adenosine receptor antagonist 3,7-dimethyl-1-propargylxanthine (DMPX). Fluorimetric measurements with fura-2 reveal that 2-chloroadenosine and ATP elicited both a Ca 2+ influx through the plasma membrane and a release from intracellular stores.