Simultaneous immunostaining method for localization of bromodeoxyuridine and calcitonin gene-related peptide

A new simultaneous double immunostaining method has been optimized to localize the DNA synthesis marker bromodeoxyuridine (BrdU) and calcitonin gene-related peptide (CGRP) in endocrine cells of Bouin's-fixed, paraffin-embedded rat lung. Nuclease pre-treatment before immunostaining is compatible...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 1992-08, Vol.40 (8), p.1121-1128
Main Authors: Montuenga, LM, Springall, DR, Gaer, J, McBride, JT, Polak, JM
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Bromodeoxyuridine - analysis
Calcitonin Gene-Related Peptide - analysis
Cell Nucleus - chemistry
Diverse techniques
Fundamental and applied biological sciences. Psychology
Immunoenzyme Techniques
Lung - chemistry
Lung - cytology
Male
Molecular and cellular biology
Rats
Rats, Inbred Strains
Specific Pathogen-Free Organisms
Staining and Labeling
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Summary:A new simultaneous double immunostaining method has been optimized to localize the DNA synthesis marker bromodeoxyuridine (BrdU) and calcitonin gene-related peptide (CGRP) in endocrine cells of Bouin's-fixed, paraffin-embedded rat lung. Nuclease pre-treatment before immunostaining is compatible with optimal tissue morphology and CGRP antigenicity preservation. Nickel-enhanced development of avidin-biotin-peroxidase staining is used to show CGRP immunoreactivity in black and alkaline phosphatase-anti-alkaline phosphatase is applied to demonstrate incorporated BrdU in red. The present methodology could be useful for studies requiring detection of incorporated BrdU in cells producing regulatory peptides or other labile antigens.
ISSN:0022-1554
1551-5044
DOI:10.1177/40.8.1377732