Comparison of adhesion of wound isolates of Staphylococcus aureus to immobilized proteins

Aims: To determine the ability of 149 clinical isolates of Staphylococcus aureus from burns, other wounds and environmental isolates to adhere to immobilized proteins. Methods and Results: The ability to bind to immobilized fibrinogen, fibronectin, laminin, collagen, IgG and lysozyme was studied usi...

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Bibliographic Details
Published in:Journal of applied microbiology 2003-01, Vol.94 (3), p.413-420
Main Authors: Elgalai, I., Foster, H. A.
Format: Article
Language:English
Subjects:
adhesins
Bacterial Adhesion - physiology
Bacteriology
Biological and medical sciences
burns
Burns - microbiology
collagen
Collagen - physiology
fibrinogen
Fibrinogen - physiology
fibronectin
Fibronectins - physiology
Fundamental and applied biological sciences. Psychology
Humans
Immunoglobulin G - physiology
Laminin - physiology
Microbiology
Muramidase - physiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Protein Binding - physiology
Proteins - physiology
Staphylococcus aureus
Staphylococcus aureus - physiology
Wound Infection - microbiology
wound infections
Wounds and Injuries - microbiology
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Summary:Aims: To determine the ability of 149 clinical isolates of Staphylococcus aureus from burns, other wounds and environmental isolates to adhere to immobilized proteins. Methods and Results: The ability to bind to immobilized fibrinogen, fibronectin, laminin, collagen, IgG and lysozyme was studied using a microtitre plate assay. The strains were very diverse. Binding to fibrinogen was most frequent, followed by fibronectin, collagen and laminin. Binding to IgG and lysozyme was weak and few strains showed strong binding. Numerical analysis showed that 65% of the strains infecting burns had similar properties and bound to fibrinogen, fibronectin, collagen and IgG. The strains infecting other wounds had more variable characteristics. Conclusions: The ability to adhere to proteins is important in wound infection, but clinical isolates were diverse in their ability to bind to the proteins tested. Burn wounds were more likely to be infected with strains showing multiple binding characteristics. Significance and Impact of the study: The study confirms the importance of adhesins in clinical infection.
ISSN:1364-5072
1365-2672
DOI:10.1046/j.1365-2672.2003.01858.x