Identification of the atrial natriuretic factor-R1C receptor subtype (B-clone) in cultured rat aortic smooth muscle cells

• Published in: FEBS letters 1992-06, Vol.305 (2), p.77-80
• Main Authors: FETHIERE, J, GRAIHLE, R, DE LEAN, A
• Format: Article
• Language: English
• Subjects: Animals; Aorta - metabolism; Atrial Natriuretic Factor - metabolism; Biological and medical sciences; Cell receptors; Cell structures and functions; Cells, Cultured; Fundamental and applied biological sciences. Psychology; Guanylate Cyclase - metabolism; Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors; Molecular and cellular biology; Muscle, Smooth, Vascular - metabolism; Natriuretic Peptide, Brain; Natriuretic Peptide, C-Type; Nerve Tissue Proteins - metabolism; Radioligand Assay; Rats; Receptors, Atrial Natriuretic Factor; Receptors, Cell Surface - metabolism; Recombinant Proteins - metabolism
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(92)80868-H

The present report demonstrates the presence in cultured rat aortic smooth muscle cells of a natriuretic factor receptor subtype with a specificity typical of the ANF-R1C (B-clone) receptor subtype. To prove the existence of this receptor subtype in this cell line we show that pCNP-(82-103) is the most potent activator of the intrinsic guanylate cyclase activity, and that [125I]pCNP-(82-103) binds to a specific receptor subtype which is insensitive to the ANF-R2 specific ligand, C-ANF. The investigation of its binding characteristics show the rank potency order of the natriuretic factors in competing for pCNP binding to be pCNP greater than pBNP greater than rANF. Furthermore it was possible to covalently photolabel this receptor subtype with underivatized]125I]pCNP and show that it is composed of a single subunit of 130 kDa with very high specificity for pCNP.