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Development of Parthenogenetic Rat Embryos
In an effort to establish cloning technology for the rat, we tested several methods (electric stimulation, treatment with ethanol or strontium) for the parthenogenetic activation of rat oocytes. We observed marked individual differences among rats of the outbred Wistar strain in their ability to yie...
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| Published in: | Biology of reproduction 2003-03, Vol.68 (3), p.829-836 |
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| Language: | English |
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| container_end_page | 836 |
| container_issue | 3 |
| container_start_page | 829 |
| container_title | Biology of reproduction |
| container_volume | 68 |
| creator | KRIVOKHARCHENKO, Alexander POPOVA, Elena ZAITSEVA, Ioulia VIL'IANOVICH, Larissa GANTEN, Detlev BADER, Michael |
| description | In an effort to establish cloning technology for the rat, we tested several methods (electric stimulation, treatment with
ethanol or strontium) for the parthenogenetic activation of rat oocytes. We observed marked individual differences among rats
of the outbred Wistar strain in their ability to yield activatable oocytes. These differences were independent of the activation
protocol and may be due to a genetic predisposition that is crucial for the parthenogenetic activation of oocytes. The activation
of oocytes was dependent upon the time between superovulation of the donor animal and the collection of the embryos. Aged
oocytes (derived about 24 h after superovulation) were more prone to activation by each method than were younger oocytes,
and some even underwent spontaneous activation without treatment and exhibited pronuclear formation and blastocyst development.
All activation methods were effective in generating parthenogenetic rat embryos, and rat parthenotes developed until implantation.
However, in general, short-term (15 min) and long-term (2 h) strontium treatment was superior to stimulation by ethanol or
electric pulse for parthenogenetic activation. These results will be helpful in achieving successful cloning in the rat. |
| doi_str_mv | 10.1095/biolreprod.102.006494 |
| format | article |
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ethanol or strontium) for the parthenogenetic activation of rat oocytes. We observed marked individual differences among rats
of the outbred Wistar strain in their ability to yield activatable oocytes. These differences were independent of the activation
protocol and may be due to a genetic predisposition that is crucial for the parthenogenetic activation of oocytes. The activation
of oocytes was dependent upon the time between superovulation of the donor animal and the collection of the embryos. Aged
oocytes (derived about 24 h after superovulation) were more prone to activation by each method than were younger oocytes,
and some even underwent spontaneous activation without treatment and exhibited pronuclear formation and blastocyst development.
All activation methods were effective in generating parthenogenetic rat embryos, and rat parthenotes developed until implantation.
However, in general, short-term (15 min) and long-term (2 h) strontium treatment was superior to stimulation by ethanol or
electric pulse for parthenogenetic activation. These results will be helpful in achieving successful cloning in the rat.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod.102.006494</identifier><identifier>PMID: 12604632</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Cloning, Organism - methods ; Electric Stimulation ; Embryology: invertebrates and vertebrates. Teratology ; Ethanol - pharmacology ; Female ; Fundamental and applied biological sciences. Psychology ; General aspects ; General aspects. Development. Fetal membranes ; Male ; Mammalian female genital system ; Microscopy, Confocal - veterinary ; Morphology. Physiology ; Oocytes - cytology ; Oocytes - physiology ; Parthenogenesis - physiology ; Pregnancy ; Rats ; Rats, Wistar - embryology ; Rats, Wistar - physiology ; Strontium - pharmacology ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2003-03, Vol.68 (3), p.829-836</ispartof><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14568395$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12604632$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KRIVOKHARCHENKO, Alexander</creatorcontrib><creatorcontrib>POPOVA, Elena</creatorcontrib><creatorcontrib>ZAITSEVA, Ioulia</creatorcontrib><creatorcontrib>VIL'IANOVICH, Larissa</creatorcontrib><creatorcontrib>GANTEN, Detlev</creatorcontrib><creatorcontrib>BADER, Michael</creatorcontrib><title>Development of Parthenogenetic Rat Embryos</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>In an effort to establish cloning technology for the rat, we tested several methods (electric stimulation, treatment with
ethanol or strontium) for the parthenogenetic activation of rat oocytes. We observed marked individual differences among rats
of the outbred Wistar strain in their ability to yield activatable oocytes. These differences were independent of the activation
protocol and may be due to a genetic predisposition that is crucial for the parthenogenetic activation of oocytes. The activation
of oocytes was dependent upon the time between superovulation of the donor animal and the collection of the embryos. Aged
oocytes (derived about 24 h after superovulation) were more prone to activation by each method than were younger oocytes,
and some even underwent spontaneous activation without treatment and exhibited pronuclear formation and blastocyst development.
All activation methods were effective in generating parthenogenetic rat embryos, and rat parthenotes developed until implantation.
However, in general, short-term (15 min) and long-term (2 h) strontium treatment was superior to stimulation by ethanol or
electric pulse for parthenogenetic activation. These results will be helpful in achieving successful cloning in the rat.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cloning, Organism - methods</subject><subject>Electric Stimulation</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Ethanol - pharmacology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>General aspects. Development. Fetal membranes</subject><subject>Male</subject><subject>Mammalian female genital system</subject><subject>Microscopy, Confocal - veterinary</subject><subject>Morphology. Physiology</subject><subject>Oocytes - cytology</subject><subject>Oocytes - physiology</subject><subject>Parthenogenesis - physiology</subject><subject>Pregnancy</subject><subject>Rats</subject><subject>Rats, Wistar - embryology</subject><subject>Rats, Wistar - physiology</subject><subject>Strontium - pharmacology</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNpFj11LwzAUhoMobk5_gtIbvRA689GmyaXM-QEDRfQ6pM3JWknbmXSW_XsDTnZ1eA8P7zkPQpcEzwmW-V3Z9M7DxvcmZjrHmGcyO0JTklOZFpSLYzTFcZsyxtkEnYXwhTHJGGWnaEIoxxlndIpuH-AHXL9poRuS3iZv2g81dP0aOhiaKnnXQ7JsS7_rwzk6sdoFuNjPGfp8XH4sntPV69PL4n6V1pQXQ0qoMVwDB8IpKyW20hhCZCkqyE0lDRWcy7ywlTGW4vhHzkthMLFSspJazWbo5q83yn1vIQyqbUIFzukO-m1QBcM5EdFqhq724LZswaiNb1rtd-rfLgLXe0CHSjvrdVc14cDF04LJ_MDVzboeGw8qtNq5WMvUOI5cKKYElewXv_JtVQ</recordid><startdate>20030301</startdate><enddate>20030301</enddate><creator>KRIVOKHARCHENKO, Alexander</creator><creator>POPOVA, Elena</creator><creator>ZAITSEVA, Ioulia</creator><creator>VIL'IANOVICH, Larissa</creator><creator>GANTEN, Detlev</creator><creator>BADER, Michael</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20030301</creationdate><title>Development of Parthenogenetic Rat Embryos</title><author>KRIVOKHARCHENKO, Alexander ; POPOVA, Elena ; ZAITSEVA, Ioulia ; VIL'IANOVICH, Larissa ; GANTEN, Detlev ; BADER, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h267t-12dd6ae6e1623b90f9dd119b8ce5dc9d2866957fcddf2060456b8d01f993b2fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cloning, Organism - methods</topic><topic>Electric Stimulation</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Ethanol - pharmacology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>General aspects. Development. Fetal membranes</topic><topic>Male</topic><topic>Mammalian female genital system</topic><topic>Microscopy, Confocal - veterinary</topic><topic>Morphology. Physiology</topic><topic>Oocytes - cytology</topic><topic>Oocytes - physiology</topic><topic>Parthenogenesis - physiology</topic><topic>Pregnancy</topic><topic>Rats</topic><topic>Rats, Wistar - embryology</topic><topic>Rats, Wistar - physiology</topic><topic>Strontium - pharmacology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KRIVOKHARCHENKO, Alexander</creatorcontrib><creatorcontrib>POPOVA, Elena</creatorcontrib><creatorcontrib>ZAITSEVA, Ioulia</creatorcontrib><creatorcontrib>VIL'IANOVICH, Larissa</creatorcontrib><creatorcontrib>GANTEN, Detlev</creatorcontrib><creatorcontrib>BADER, Michael</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KRIVOKHARCHENKO, Alexander</au><au>POPOVA, Elena</au><au>ZAITSEVA, Ioulia</au><au>VIL'IANOVICH, Larissa</au><au>GANTEN, Detlev</au><au>BADER, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of Parthenogenetic Rat Embryos</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2003-03-01</date><risdate>2003</risdate><volume>68</volume><issue>3</issue><spage>829</spage><epage>836</epage><pages>829-836</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>In an effort to establish cloning technology for the rat, we tested several methods (electric stimulation, treatment with
ethanol or strontium) for the parthenogenetic activation of rat oocytes. We observed marked individual differences among rats
of the outbred Wistar strain in their ability to yield activatable oocytes. These differences were independent of the activation
protocol and may be due to a genetic predisposition that is crucial for the parthenogenetic activation of oocytes. The activation
of oocytes was dependent upon the time between superovulation of the donor animal and the collection of the embryos. Aged
oocytes (derived about 24 h after superovulation) were more prone to activation by each method than were younger oocytes,
and some even underwent spontaneous activation without treatment and exhibited pronuclear formation and blastocyst development.
All activation methods were effective in generating parthenogenetic rat embryos, and rat parthenotes developed until implantation.
However, in general, short-term (15 min) and long-term (2 h) strontium treatment was superior to stimulation by ethanol or
electric pulse for parthenogenetic activation. These results will be helpful in achieving successful cloning in the rat.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>12604632</pmid><doi>10.1095/biolreprod.102.006494</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biology of reproduction, 2003-03, Vol.68 (3), p.829-836 |
| issn | 0006-3363 1529-7268 |
| language | eng |
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| source | Oxford Journals Online |
| subjects | Animals Biological and medical sciences Cloning, Organism - methods Electric Stimulation Embryology: invertebrates and vertebrates. Teratology Ethanol - pharmacology Female Fundamental and applied biological sciences. Psychology General aspects General aspects. Development. Fetal membranes Male Mammalian female genital system Microscopy, Confocal - veterinary Morphology. Physiology Oocytes - cytology Oocytes - physiology Parthenogenesis - physiology Pregnancy Rats Rats, Wistar - embryology Rats, Wistar - physiology Strontium - pharmacology Vertebrates: reproduction |
| title | Development of Parthenogenetic Rat Embryos |
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