Persistence and expression of Microplitis demolitor polydnavirus in Pseudoplusia includens

Persistence and expression of Microplitis demolitor polydnavirus (MdPDV) was examined in parasitized and virus-injected Pseudoplusia includens larvae. Viral DNA persisted in P. includens larvae for 6 days, but no increase in the amount of viral DNA present was detected. Viral transcripts were observ...

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Bibliographic Details
Published in:Journal of general virology 1992-07, Vol.73 (7), p.1627-1635
Main Authors: Strand, M.R, McKenzie, D.I, Grassl, V, Dover, B.A, Aiken, J.M
Format: Article
Language:English
Subjects:
Animals
Baculoviridae - isolation & purification
Biological and medical sciences
Braconidae
DNA
DNA, Viral - analysis
Female
Fundamental and applied biological sciences. Psychology
Hymenoptera
Larva - microbiology
Lepidoptera
Lepidoptera - microbiology
Microbiology
Microplitis demolitor
Noctuidae
Polydnaviridae
polydnavirus
Pseudoplusia includens
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
RNA, Messenger - analysis
RNA, Viral - analysis
Virology
virus expression
virus persistance
Wasps - microbiology
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Summary:Persistence and expression of Microplitis demolitor polydnavirus (MdPDV) was examined in parasitized and virus-injected Pseudoplusia includens larvae. Viral DNA persisted in P. includens larvae for 6 days, but no increase in the amount of viral DNA present was detected. Viral transcripts were observed in parasitized and virus-injected larvae 4 h post-parasitism and expression continued for 6 days. When specific host tissues were examined, more viral DNA and RNA was detected in haemocytes than in the gut, nervous system and fat body. 3P-labelled MdPDV DNA hybridized to approximately six different size classes of mRNAs on Northern blots of RNA from haemocytes of parasitized larvae. MdPDV transcription was first detected in haemocytes at 4 h post-parasitism and continued for 6 days. Similar transcripts were observed in haemocytes from larvae that had been injected with calyx fluid or MdPDV plus venom. First-strand cDNA probes of haemocyte-specific MdPDV transcripts hybridized to only certain MdPDV viral DNAs. suggesting that only part of the MdPDV genome is expressed in this host cell type.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-73-7-1627