Chitosan enhances platelet adhesion and aggregation

In this study, chitosan (MW=50,000) was tested for its enhancing platelet activity in rabbit platelet suspensions and the possible mechanisms involved were further investigated. Our results showed that after initial (5 min) and long-term (30 min) contact of platelets with chitosan, the platelet adhe...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2003-03, Vol.302 (3), p.480-483
Main Authors: Chou, Tz-Chong, Fu, Earl, Wu, Chang-Jer, Yeh, Jeng-Hsien
Format: Article
Language:English
Subjects:
Aggregation
Animals
Calcium - metabolism
Cell Adhesion - drug effects
Chitin - analogs & derivatives
Chitin - metabolism
Chitin - pharmacology
Chitosan
Dose-Response Relationship, Drug
Flow Cytometry
Fluorescent Dyes - pharmacology
Glycoprotein IIb/IIIa
Integrin beta3 - biosynthesis
Intracellular calcium level
Platelet adhesion
Platelet Aggregation - drug effects
Platelet Glycoprotein GPIIb-IIIa Complex - metabolism
Platelet Membrane Glycoprotein IIb - biosynthesis
Rabbits
Temperature
Time Factors
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Summary:In this study, chitosan (MW=50,000) was tested for its enhancing platelet activity in rabbit platelet suspensions and the possible mechanisms involved were further investigated. Our results showed that after initial (5 min) and long-term (30 min) contact of platelets with chitosan, the platelet adhesion to chitosan-coated microtiter plates was dose-dependently increased compared to that of solvent control. Similarly, chitosan also dose-dependently increased the platelet aggregation and the intracellular free Ca 2+ rise of Fura-2-AM loaded platelets. Additionally, in the presence of FITC-labeled anti-CD41/CD61, chitosan significantly enhanced the expression of platelet glycoprotein IIb/IIIa complex assayed by a flow cytometer. It is concluded that chitosan is an effective inducer for platelet adhesion and aggregation and the mechanisms of action of chitosan may be associated, at least partly, with the increasing [Ca 2+] i mobilization and enhancing expression of GPIIb/IIIa complex on platelet membrane surfaces.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(03)00173-6