The broad diversity of neutral and sialylated oligosaccharides derived from human salivary mucins

Mucin glycopeptides were prepared from the salivary mucins of 20 healthy donors with blood group O. The carbohydrate chains of the high-molecular-weight mucins were released by alkaline borohydride treatment. Neutral and monosialylated oligosaccharide-alditols were purified by ion-exchange chromatog...

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Bibliographic Details
Published in:Biochemistry (Easton) 1992-07, Vol.31 (26), p.6152-6165
Main Authors: Klein, Andre, Carnoy, Christophe, Wieruszeski, Jean Michel, Strecker, Gerard, Strang, Anne Marie, Van Halbeek, Herman, Roussel, Philippe, Lamblin, Genevieve
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Carbohydrate Conformation
Carbohydrate Sequence
Chromatography, Gel
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Fundamental and applied biological sciences. Psychology
Glycoproteins
Humans
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Molecular Structure
Mucins - chemistry
Oligosaccharides - chemistry
Oligosaccharides - isolation & purification
Pronase - metabolism
Proteins
Saliva - chemistry
Spectrometry, Mass, Fast Atom Bombardment
Sugar Alcohols - chemistry
Sugar Alcohols - isolation & purification
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Summary:Mucin glycopeptides were prepared from the salivary mucins of 20 healthy donors with blood group O. The carbohydrate chains of the high-molecular-weight mucins were released by alkaline borohydride treatment. Neutral and monosialylated oligosaccharide-alditols were purified by ion-exchange chromatography, gel filtration, and HPLC. The structures of the oligosaccharide-alditols were determined by high-resolution 1H-NMR spectroscopy in combination with fast atom bombardment mass spectrometry and methylation analysis. Thirty-seven oligosaccharide-alditols were characterized and illustrate the extreme diversity of the salivary mucins carbohydrate chains. This diversity might represent a mosaic of bacterial adhesion sites and be involved in the early events of the nonimmune defense of the oral cavity. Among these 37 oligosaccharide-alditols, 31 have not been previously described in human saliva and five of these are novel structures: [formula: see text]
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00141a028