Ellipsometric immunosensors for the determination of γ-interferon and human serum albumin

Ellipsometric immunosensors for γ-interferon (γ-INF) and human serum albumin (HSA) have been developed using γ-INF and HSA covalently immobilized onto silicon plates. Detection is achieved by measuring the adsorption decrease (Γ, mg m−2) of monoclonal immunoglobulin G against γ-INF or immunoglobulin...

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Bibliographic Details
Published in:Biosensors & bioelectronics 1992, Vol.7 (4), p.305-308
Main Authors: Ruzgas, T.A., Razumas, V.J., Kulys, J.J.
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal
Biological and medical sciences
Biosensing Techniques
Biosensors
Biotechnology
ellipsometric immunosensor
Fundamental and applied biological sciences. Psychology
human serum albumin
Humans
immobilization
Immunoglobulin G - immunology
Interferon-gamma - analysis
Methods. Procedures. Technologies
Serum Albumin - analysis
silicon surfaces
Surface Properties
Various methods and equipments
γ-interferon
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Summary:Ellipsometric immunosensors for γ-interferon (γ-INF) and human serum albumin (HSA) have been developed using γ-INF and HSA covalently immobilized onto silicon plates. Detection is achieved by measuring the adsorption decrease (Γ, mg m−2) of monoclonal immunoglobulin G against γ-INF or immunoglobulin fraction of rabbit blood serum against HSA when the concentration of antigen in mixtures of antibody-antigen is increased. The time of analysis is 50 min, the detection limit of γ-INF is 15 nm (sensitivity −0.01 mg m−2 nm−1) and of HSA 2.5 nm (−0.06 mg m−2 nm−1).
ISSN:0956-5663
1873-4235
DOI:10.1016/0956-5663(92)87009-E