Occurrence of prohormone convertase-like substances in the neural complex cells of the ascidian Halocynthia roretzi

• Published in: General and comparative endocrinology 2003-03, Vol.131 (1), p.32-37
• Main Authors: Kawahara, G., Terakado, K., Tanaka, S., Kikuyama, S.
• Format: Article
• Language: English
• Subjects: Adenohypophysis; Adrenocorticotropic Hormone - metabolism; Animals; Ascidian; Blotting, Western; Cerebral ganglion; Dorsal strand; Furin; Ganglia, Invertebrate - cytology; Ganglia, Invertebrate - metabolism; Halocynthia roretzi; Immunohistochemistry; Isoenzymes - metabolism; Marine; Microscopy, Immunoelectron; Neurons - metabolism; Prohormone convertase; Subtilisins - metabolism; Urochordata - metabolism
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/S0016-6480(02)00608-1

Our previous study on the distribution of adrenocorticotropin (ACTH)-like substances in the neural complex (cerebral ganglion, dorsal strand, and neural gland) of an ascidian Halocynthia roretzi revealed that some of the cells in the cerebral ganglion and the cells scattered along the dorsal strand were immunopositive with antiserum against ACTH. In order to ascertain whether these cells are equipped with prohormone convertases, we performed immunohistochemical studies on the neural complex by using antisera against PC1 and PC2. A considerable number of cells around the dorsal strand and a few cells in the neural ganglion were immunopositive with PC1 and/or PC2 antibodies. Immunoelectron microscopic study demonstrated that some granulated cells situated in the cerebral ganglion and along the dorsal strand contained PC1- or PC2-like substances within their secretory granules. Western blot analysis revealed the presence of 66-kDa PC1-like and 70-kDa PC2-like substances in the neural complex. Moreover, immunostaining of consecutive sections showed that the majority of the cells containing PC1- and/or PC2-like substances corresponded to the cells immunoreactive with antisera against ACTH and CLIP but not to those immunoreactive with an antiserum against PRL. Cells belonging to the neural gland neither contained electron-dense granules nor showed immunoreactivity with any antisera employed in this experiment. The possibility that some of the cells situated in the cerebral ganglion and along the dorsal strand are progenitors of vertebrate adenohypophyseal cells is discussed.