G protein beta 3 subunit 825T allele carriage and risk of coronary artery disease

• Published in: Atherosclerosis 2003-03, Vol.167 (1), p.135-139
• Main Authors: von Beckerath, Nicolas, Schusterschitz, Yasmin, Koch, Werner, Grießer, Korinna, Mehilli, Julinda, Gorchakova, Olga, Schömig, Albert, Kastrati, Adnan
• Format: Article
• Language: English
• Subjects: Aged; Alleles; Base Sequence; Biological and medical sciences; Cardiology. Vascular system; Case-Control Studies; Coronary artery disease; Coronary Artery Disease - genetics; Coronary Artery Disease - physiopathology; Coronary heart disease; Female; G proteins; Genetic Predisposition to Disease; Genotype; Heart; Heterotrimeric GTP-Binding Proteins - genetics; Heterozygote; Humans; Logistic Models; Male; Medical sciences; Middle Aged; Molecular Sequence Data; Multivessel disease, genetics; Polymerase Chain Reaction - methods; Polymorphism, Genetic; Probability; Reference Values; Risk Assessment; Severity of Illness Index
• ISSN: 0021-9150; 1879-1484
• DOI: 10.1016/S0021-9150(02)00422-7

C825T polymorphism in the G protein β3 subunit gene (GNB3) is associated with increased transmembrane signal transduction via adenylyl cyclase inhibiting G (G i) proteins. We tested whether GNB3 C825T is associated with an increased risk of coronary artery disease (CAD). Genotypes were determined with polymerase chain reaction and allele-specific fluorogenic probes. Angiographically examined, consecutive patients ( n=998) with CAD and angiographically examined, sex- and age-matched controls ( n=340) with no evidence of CAD were studied. The proportion of T allele carriers was significantly higher in the group with CAD compared with the control group (55.6 vs. 48.5; P=0.02). T allele carriage was associated with a 33% increase in the unadjusted risk (OR 1.33 [95% confidence interval, 1.04–1.70]) and a 37% increase in the adjusted risk (OR from the multivariate model 1.37 [95% CI, 1.06–1.76]) for CAD. Moreover, an increase in T allele carriage was associated with an increase in disease severity ( P=0.006; test for trend). The strongest association was observed between T allele carriage and three-vessel disease (unadjusted OR 1.47 [95% CI, 1.10–1.96]). Thus, carrying this allele is associated with the presence as well as the severity of CAD.