A reagentless electrochemical biosensor based on a protein scaffold

Apo-myoglobin, labeled with the environmentally sensitive redox probe RuII(NH3)4(1,10-phenanthroline-5-maleimide)2+, was immobilized onto gold electrodes modified with 11-mercaptoundecanoic acid and subsequently labeled with biotin; avidin binding to the immobilized biotin was specifically and quant...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2003-02 (3), p.338-339
Main Authors: Jhaveri, Sulay D, Mauro, J Matthew, Goldston, Harold M, Schauer, Caroline L, Tender, Leonard M, Trammell, Scott A
Format: Article
Language:English
Subjects:
Animals
Apoproteins - chemistry
Avidin - chemistry
Biosensing Techniques - methods
Biotin - chemistry
Electrochemistry
Electrodes
Molecular Probe Techniques
Myoglobin - chemistry
Organometallic Compounds
Oxidation-Reduction
Protein Engineering
Ruthenium - chemistry
Whales
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Summary:Apo-myoglobin, labeled with the environmentally sensitive redox probe RuII(NH3)4(1,10-phenanthroline-5-maleimide)2+, was immobilized onto gold electrodes modified with 11-mercaptoundecanoic acid and subsequently labeled with biotin; avidin binding to the immobilized biotin was specifically and quantitatively detected by a change in cyclic voltammetry of the co-immobilized probe.
ISSN:1359-7345
DOI:10.1039/b209452e