Separation of proteins on polymeric stationary phases grafted with various amine groups

Six polymeric stationary phases with various amine groups were prepared by surface grafting of glycidyl methacrylate on silica gel surface and its subsequent amination. The six kinds of amines, namely, triethylamine, diethylamine, ethylenediamine (EDA), hexaethylenediamine (HEDA), diethylenetriamine...

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Bibliographic Details
Published in:Journal of Chromatography A 2003-02, Vol.987 (1), p.323-330
Main Authors: Choi, Seong-Ho, Hwang, Young-Mi, Lee, Kwang-Pill
Format: Article
Language:English
Subjects:
Amines
Amines - chemistry
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Glycidyl methacrylate
Proteins
Proteins - isolation & purification
Spectroscopy, Fourier Transform Infrared
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Summary:Six polymeric stationary phases with various amine groups were prepared by surface grafting of glycidyl methacrylate on silica gel surface and its subsequent amination. The six kinds of amines, namely, triethylamine, diethylamine, ethylenediamine (EDA), hexaethylenediamine (HEDA), diethylenetriamine (DETA) and triethylenetetraamine (TETA) were used in this study. The separation of bovine serum albumin (BSA) and chicken egg albumin (CEA) on the polymeric stationary phase with various amines was investigated. The affinity degree of BSA was higher than CEA for the EDA, HEDA and DETA columns, whereas the affinity degree of CEA was higher than BSA for the TETA column.
ISSN:0021-9673
DOI:10.1016/S0021-9673(02)01840-X