Fast and single solid phase fluorescence spectroscopic batch procedure for (acetyl) salicylic acid determination in drug formulations

• Published in: Journal of pharmaceutical and biomedical analysis 2003-03, Vol.31 (3), p.439-446
• Main Authors: Ortega Algar, S, Ramos Martos, N, Molina Dı́az, A
• Format: Article
• Language: English
• Subjects: (Acetyl) salicylic acid; Algorithms; Analysis; Anti-Inflammatory Agents, Non-Steroidal - analysis; Aspirin - analysis; Biological and medical sciences; Caffeine - chemistry; Capsules; Chromatography, DEAE-Cellulose; Chromatography, Ion Exchange; Codeine - chemistry; Drug Combinations; Drug formulations; General pharmacology; Hydrogen-Ion Concentration; Indicators and Reagents; Medical sciences; Pharmacology. Drug treatments; Solid-phase fluorimetry; Spectrometry, Fluorescence; Tablets
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/S0731-7085(02)00719-7

A solid phase fluorescence spectroscopic batch procedure for (acetyl) salicylic acid in drug formulations have been developed. The procedure is based on the sorption of salicylic acid (SA) on Sephadex DEAE A-25 anion exchanger gel (100 mg) by equilibration from an aqueous solution (10 or 25 ml) for 5 min; the equilibrated gel is transferred into an 1 mm quartz cell and the native fluorescence of SA sorbed on it is directly measured ( λ ex=297 nm; λ em=405 nm). Good linearity was found in the 10–200 and 5–100 μg l −1 ranges (for 10 and 25 ml sample volume, respectively) with R.S.D. (%) of 2.8 and 1.1. The procedure was successfully applied to the determination of acetyl salicylic acid (ASA) in drug formulations after alkaline hydrolysis to yield SA.