Brassinosteroid functions in a broad range of disease resistance in tobacco and rice

• Published in: The Plant journal : for cell and molecular biology 2003-03, Vol.33 (5), p.887-898
• Main Authors: Nakashita, Hideo, Yasuda, Michiko, Nitta, Takako, Asami, Tadao, Fujioka, Shozo, Arai, Yuko, Sekimata, Katsuhiko, Takatsuto, Suguru, Yamaguchi, Isamu, Yoshida, Shigeo
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; biosynthesis; blast disease; blight; brassinolide; brassinosteroid; Brassinosteroids; Cholestanols - metabolism; Cholestanols - pharmacology; disease resistance; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; fungi; Fungi - physiology; gene expression; growth and development; hormonal regulation; Immunity, Innate - drug effects; innate immunity; leaves; Magnaporthe grisea; Nicotiana - drug effects; Nicotiana - immunology; Nicotiana - metabolism; Oidium; Oryza - drug effects; Oryza - immunology; pathogens; Pathology, epidemiology, host-fungus relationships. Damages, economic importance; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - microbiology; Plant Diseases - virology; plant growth; protective effect; Pseudomonas - physiology; Pseudomonas syringae pv. tabaci; rice; salicylic acid; Salicylic Acid - metabolism; Steroids, Heterocyclic - metabolism; Steroids, Heterocyclic - pharmacology; systemic acquired resistance; Time Factors; tobacco; Tobacco mosaic virus; Tobacco Mosaic Virus - physiology; transgenic plants; Triazoles - pharmacology; Xanthomonas oryzae pv. oryzae
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1046/j.1365-313X.2003.01675.x

Brassinolide (BL), considered to be the most important brassinosteroid (BR) and playing pivotal roles in the hormonal regulation of plant growth and development, was found to induce disease resistance in plants. To study the potentialities of BL activity on stress responding systems, we analyzed its ability to induce disease resistance in tobacco and rice plants. Wild‐type tobacco treated with BL exhibited enhanced resistance to the viral pathogen tobacco mosaic virus (TMV), the bacterial pathogen Pseudomonas syringae pv. tabaci (Pst), and the fungal pathogen Oidium sp. The measurement of salicylic acid (SA) in wild‐type plants treated with BL and the pathogen infection assays using NahG transgenic plants indicate that BL‐induced resistance does not require SA biosynthesis. BL treatment did not induce either acidic or basic pathogenesis‐related (PR) gene expression, suggesting that BL‐induced resistance is distinct from systemic acquired resistance (SAR) and wound‐inducible disease resistance. Analysis using brassinazole 2001, a specific inhibitor for BR biosynthesis, and the measurement of BRs in TMV‐infected tobacco leaves indicate that steroid hormone‐mediated disease resistance (BDR) plays part in defense response in tobacco. Simultaneous activation of SAR and BDR by SAR inducers and BL, respectively, exhibited additive protective effects against TMV and Pst, indicating that there is no cross‐talk between SAR‐ and BDR‐signaling pathway downstream of BL. In addition to the enhanced resistance to a broad range of diseases in tobacco, BL induced resistance in rice to rice blast and bacterial blight diseases caused by Magnaporthe grisea and Xanthomonas oryzae pv. oryzae, respectively. Our data suggest that BDR functions in the innate immunity system of higher plants including dicotyledonous and monocotyledonous species.