Estrogen receptor alpha mRNA in human skeletal muscles

To explain the effect of estrogen on skeletal muscle, the presence of estrogen receptor alpha mRNA (ERalpha mRNA) was investigated in human skeletal muscle. The highly sensitive technique of nested reverse transcriptase-polymerase chain reaction (nested RT-PCR) was applied on a variety of tissue sam...

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Bibliographic Details
Published in:Medicine and science in sports and exercise 2003-03, Vol.35 (3), p.439-443
Main Authors: Lemoine, Sophie, Granier, Pascale, Tiffoche, Christophe, Rannou-Bekono, Francoise, Thieulant, Marie-Lise, Delamarche, Paul
Format: Article
Language:English
Subjects:
Adult
DNA, Complementary - metabolism
Electrophoresis, Agar Gel
Estrogen Receptor alpha
Female
Humans
Male
Muscle, Skeletal - chemistry
Muscle, Skeletal - metabolism
Myometrium - chemistry
Myometrium - metabolism
Receptors, Estrogen - biosynthesis
Receptors, Estrogen - genetics
Reference Values
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Space life sciences
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Summary:To explain the effect of estrogen on skeletal muscle, the presence of estrogen receptor alpha mRNA (ERalpha mRNA) was investigated in human skeletal muscle. The highly sensitive technique of nested reverse transcriptase-polymerase chain reaction (nested RT-PCR) was applied on a variety of tissue samples of both sexes: women (deltoid, pectoral, and uterus muscles) (N= 3) and men (deltoid muscle) (N= 3). The total ribonucleic acid was isolated from each tissue sample, reverse transcribed in a thermocycler, and nested PCR was then performed with specific primers. The by-products were analyzed by agarose gel electrophoresis. Internal standard 28S was simultaneously amplified. The ERalpha mRNA level was quantitated by using the ERalpha mRNA/28S mRNA ratio. The expected 204-bp product corresponding to ERalpha was amplified in all tested tissue samples, i.e., deltoid, pectoral, and uterine muscles from women and deltoid muscle from men. The ERalpha mRNA/28S mRNA ratios indicating the receptor expression levels in deltoid muscle from men and women were 0.945 +/- 0.393 (mean +/- SD) (N= 3) and 0.973 +/- 0.136 (mean +/- SD) (N= 2), respectively. In conclusion, the nested RT-PCR technique identified the presence of transcript encoding ERalpha mRNA in human skeletal muscles. Semi-quantification did not reveal gender difference.
ISSN:0195-9131
DOI:10.1249/01.MSS.0000053654.14410.78