Molecular evolution and phylogeny of dengue type 4 virus in the caribbean

We sequenced the E gene and adjacent prM/M and NS1 junctions (1940 bp) of 48 Dengue-4 (DEN-4) isolates collected between 1981 and 1999 from 8 Caribbean islands and from 7 South and Central American countries. Phylogenetic analysis confirms a single introduction in the early 1980s and a high degree o...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2003-02, Vol.306 (1), p.126-134
Main Authors: Foster, Jerome E, Bennett, Shannon N, Vaughan, Helen, Vorndam, Vance, McMillan, W.Owen, Carrington, Christine V.F
Format: Article
Language:English
Subjects:
Caribbean
Caribbean Region
DEN-4
Dengue
Dengue - virology
Dengue Virus - genetics
DNA, Viral - analysis
Envelope
Evolution
Evolution, Molecular
Humans
Molecular Sequence Data
NS1
Phylogeny
Sequence Analysis, DNA
Viral Envelope Proteins - genetics
Viral Envelope Proteins - metabolism
Viral Nonstructural Proteins - genetics
Viral Nonstructural Proteins - metabolism
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Summary:We sequenced the E gene and adjacent prM/M and NS1 junctions (1940 bp) of 48 Dengue-4 (DEN-4) isolates collected between 1981 and 1999 from 8 Caribbean islands and from 7 South and Central American countries. Phylogenetic analysis confirms a single introduction in the early 1980s and a high degree of gene flow resulting in a pattern of evolution defined more by time period than geographic origin, especially within the Caribbean basin. A modern Caribbean clade consisting of four distinct lineages has arisen, comprised of isolates from Caribbean islands and nearby regions of South America. This clade is defined by three amino acid substitutions in the E (aa 163 and 351) and NS1 (aa 52) proteins. These findings highlight the importance of migration and gene flow in dengue viral change and suggest that efforts to understand disease dynamics in the Caribbean basin need to focus at regional, rather than local scales.
ISSN:0042-6822
1096-0341
DOI:10.1016/S0042-6822(02)00033-8