Anti-basal ganglia antibody abnormalities in Sydenham chorea

Anti-basal ganglia antibodies (ABGA) were measured in nine children with Sydenham chorea (SC) and compared to nine controls. Enzyme-linked immunosorbent assay (ELISA) and Western blot (WB) methods were used to detect ABGA against supernatant (S1), pellet, and synaptosomal preparations from adult and...

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Bibliographic Details
Published in:Journal of neuroimmunology 2003-03, Vol.136 (1), p.154-161
Main Authors: Singer, Harvey S, Loiselle, Christopher R, Lee, Olivia, Garvey, Marjorie A, Grus, Franz H
Format: Article
Language:English
Subjects:
Adolescent
Anti-basal ganglia antibodies
Autoantibodies - blood
Autoantibodies - immunology
Autoimmune Diseases of the Nervous System - blood
Autoimmune Diseases of the Nervous System - immunology
Basal Ganglia - immunology
Basal Ganglia - pathology
Basal Ganglia - physiopathology
Blotting, Western
Child
Child, Preschool
Chorea - blood
Chorea - immunology
Discriminant Analysis
ELISA
Enzyme-Linked Immunosorbent Assay
Female
Humans
Male
Molecular Weight
Sydenham chorea
Synaptosomes - immunology
Synaptosomes - metabolism
Western blot
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Summary:Anti-basal ganglia antibodies (ABGA) were measured in nine children with Sydenham chorea (SC) and compared to nine controls. Enzyme-linked immunosorbent assay (ELISA) and Western blot (WB) methods were used to detect ABGA against supernatant (S1), pellet, and synaptosomal preparations from adult and pediatric postmortem caudate, putamen, and globus pallidus. ELISA optical density (OD) values were higher in SC patients than controls across all preparations, but did not reach a level of significance. Although WB identified multiple bands in all subjects, discriminant analysis showed that the mean binding patterns of SC patients were significantly different from control, most notably in the caudate S1 fraction (Wilks' λ=0.011, p
ISSN:0165-5728
1872-8421
DOI:10.1016/S0165-5728(03)00034-1