Correlation between CMV genotypes, multiple infections with herpesviruses (HHV-6, 7) and development of CMV disease in kidney recipients in Kuwait

The possible correlation between cytomegalovirus, human herpesvirus types 6, 7 and cytomegalovirus-related clinical symptoms was studied in kidney transplant patients in Kuwait. Cytomegalovirus infection was diagnosed using the pp65 antigenemia assay. DNA of cytomegalovirus was detected by nested po...

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Bibliographic Details
Published in:FEMS immunology and medical microbiology 2003-03, Vol.35 (2), p.125-130
Main Authors: Pacsa, A.S, Essa, S, Voevodin, A, El-Shazly, A, Kazak, H, Nampoory, M.R.N, Johny, K.V, Said, T, Al-Nakib, W
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Biological and medical sciences
CMV genotypes
Cytomegalovirus - classification
Cytomegalovirus - genetics
Cytomegalovirus Infections - virology
DNA, Viral - blood
Female
Genotype
Herpesvirus 6, Human - genetics
Herpesvirus 7, Human - genetics
HHV-6
HHV-7
Human viral diseases
Humans
Infectious diseases
Kidney transplant recipients
Kidney Transplantation - adverse effects
Male
Medical sciences
Middle Aged
Miscellaneous
Roseolovirus Infections - virology
Viral diseases
Viral Envelope Proteins - genetics
Viral Load
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Summary:The possible correlation between cytomegalovirus, human herpesvirus types 6, 7 and cytomegalovirus-related clinical symptoms was studied in kidney transplant patients in Kuwait. Cytomegalovirus infection was diagnosed using the pp65 antigenemia assay. DNA of cytomegalovirus was detected by nested polymerase chain reaction (nested-PCR). PCR was also used to amplify the genes coding for structural proteins of human herpesvirus-6 (240 bp) and human herpesvirus-7 (186 bp). Glycoprotein B genotypes of cytomegalovirus were determined by restriction fragment length polymorphism. The average number of cells positive for cytomegalovirus pp65 antigen showed a steady increase with the severity of the cytomegalovirus-related symptoms. Furthermore, cytomegalovirus pp65 antigen positivity was significantly more frequent among recipients of cadaver kidney (45.5%) than among those who received live related kidneys (22.6%). Cytomegalovirus gB genotype 1 was detected more frequently ( P
ISSN:0928-8244
1574-695X
DOI:10.1016/S0928-8244(03)00006-3