Complete nucleotide sequence and molecular characterization of two lytic Staphylococcus aureus phages: 44AHJD and P68

The first complete nucleotide sequences of two lytic Staphylococcus aureus double stranded DNA phages, 44AHJD (16 784 bp) and P68 (18 227 bp), are reported. Both are small isometric phages, with short, non-contractile tails and a pre-neck appendage. Based on their morphology, their genome size, the...

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Bibliographic Details
Published in:FEMS microbiology letters 2003-02, Vol.219 (2), p.275-283
Main Authors: Vybiral, Dietmar, Takáč, Marian, Loessner, Martin, Witte, Angela, von Ahsen, Uwe, Bläsi, Udo
Format: Article
Language:English
Subjects:
Bacteriology
bacteriophages
Bacteriophages - classification
Bacteriophages - genetics
Bacteriophages - ultrastructure
Base Sequence
Biological and medical sciences
DNA
DNA fragmentation
DNA Replication
DNA, Viral
DNA-directed DNA polymerase
electrophoresis
Evolution, Molecular
Fundamental and applied biological sciences. Psychology
Lytic phage
Microbiology
Miscellaneous
Models, Genetic
Molecular Sequence Data
Nucleotide sequence
nucleotide sequences
nucleotides
operon
Podoviridae
Promoter Regions, Genetic
Sequence Alignment
Signal Transduction
Staphylococcus aureus
Staphylococcus aureus - isolation & purification
Staphylococcus aureus - virology
structural genes
Terminator Regions, Genetic
Virology
Virus Assembly
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Summary:The first complete nucleotide sequences of two lytic Staphylococcus aureus double stranded DNA phages, 44AHJD (16 784 bp) and P68 (18 227 bp), are reported. Both are small isometric phages, with short, non-contractile tails and a pre-neck appendage. Based on their morphology, their genome size, the similarity of the encoded gene products, the type of infection and on the possession of a type B DNA polymerase, 44AHJD and P68 are allocated to the order Caudovirales, family Podoviridae, genus ‘φ29-like phages’. The genome of 44AHJD differs from that of P68 by a deletion spanning nucleotides 10 091 to 11 531 of the P68 genome. The electrophoretic analysis of the terminal DNA fragments of P68 DNA and P68 DNA protein complex suggested the presence of a terminal protein at either DNA end. In contrast to the lysis cassette of the φ29-like phages, which is located at the end of the late operon, the lysis cassette of 44AHJD and P68 is located within the structural genes.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(03)00028-4