Growth of a Human Yolk SAC Tumor Cell Line with Yolk SAC-Derived Functions in Selenium-Supplemented Chemically Defined Synthetic Medium

• Published in: In Vitro Cellular & Developmental Biology - Animal 1992-06, Vol.28A (6), p.449-454
• Main Authors: Kiyoshi Ohkawa, Takashi Hatano, Naoko Takizawa, Kazue Shinmoto, Kyosuke Yamada, Makoto Matsuda, Koji Takada, Tsukada, Yutaka
• Format: Article
• Language: English
• Subjects: alpha-Fetoproteins - metabolism; beta 2-Microglobulin - metabolism; Biological and medical sciences; Biotechnology; Blood proteins; Carcinoembryonic Antigen - metabolism; Cell culture techniques; Cell Division - drug effects; Cell growth; Cell lines; Culture Media - chemistry; Culture Media - pharmacology; Cultured cells; Electrophoresis; Epidermal Growth Factor - metabolism; Eukaryotic cell cultures; Fucosyltransferases - analysis; Fucosyltransferases - metabolism; Fundamental and applied biological sciences. Psychology; Germ cells; Glycosyltransferases - metabolism; Glycosyltransferases - physiology; Human growth; Humans; Immunohistochemistry; Lectins; Male; Mesonephroma; Mesonephroma - enzymology; Mesonephroma - metabolism; Mesonephroma - pathology; Methods. Procedures. Technologies; Phosphopyruvate Hydratase - metabolism; Platelet-Derived Growth Factor - metabolism; Polyamines - metabolism; Selenium - analysis; Selenium - pharmacology; Serum Albumin, Bovine - analysis; Serum Albumin, Bovine - pharmacology; Testicular Neoplasms - enzymology; Testicular Neoplasms - metabolism; Testicular Neoplasms - pathology; Transferrin - metabolism; Tumor cell line; Tumor Cells, Cultured - enzymology; Tumor Cells, Cultured - metabolism; Tumor Cells, Cultured - pathology
• ISSN: 0883-8364; 2327-431X; 1543-706X
• DOI: 10.1007/BF02634050

A human yolk sac tumor cell line, TG1, which was established from a testicular yolk sac tumor, was found to replicate continuously in a chemically defined medium supplemented with$Na_{2}SeO_3$(ISRPMI). TG1 produced several plasma proteins and growth factors: albumin, alpha-fetoprotein (AFP), ferritin, carcinoembryonic antigen, beta-2-microglobulin, polyamine, neuron specific enolase, tissue polypeptide antigen, transferrin (Tf), epidermal growth factor, and platelet derived growth factor. By analysis of lentil lectin (LcHA)-affinity electrophoresis, to examine the microheterogeneity of carbohydrate chains of synthetic glycoproteins, TG1 cells cultured with ISRPMI produced only LcHA reactive Tf and AFP based on core fucose attached to asparagine-linked N-acetylglucosamine residues instead of LcHA-nonreactive Tf and AFP produced by TG1 cells cultured with fetal bovine serum (FBS)-containing medium,$\alpha 1-6$Fucosyltransferase activity was significantly greater in the TG1 cells cultured with ISRPMI ($39.9 \pm 1.5 pmol \cdot h^{-1} \cdot mg^{-1}$protein) than cultured with FBS-containing media ($18.2 \pm 1.2 pmol \cdot h^{-1} \cdot mg^{-1}$protein). These results have indicated that the selective increase of$\alpha 1-6$fucosyltransferase occurred when the cells were cultured with the FBS-free synthetic media.