Low-Inoculum Model of Surgical Wound Infection

A model of surgical wound infection that uses low inocula of bacteria and closely simulates clinical infection involved inoculating suspensions of Staphylococcus aureus and dextran microbeads into intermuscular sites on the dorsum of guinea pigs, harvesting lesions at 72–96 h, identifying as a posit...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of infectious diseases 1992-08, Vol.166 (2), p.393-399
Main Authors: Kaiser, Allen B., Kernodle, Douglas S., Parker, Robert A.
Format: Article
Language:English
Subjects:
Abscess - microbiology
Abscess - prevention & control
Ampicillin - administration & dosage
Ampicillin - pharmacokinetics
Ampicillin - therapeutic use
Animal models
Animals
Antibiotics
Antimicrobials
Bacteria
Biological and medical sciences
Cefazolin - administration & dosage
Cefazolin - pharmacokinetics
Cefazolin - therapeutic use
Disease Models, Animal
Female
Guinea Pigs
Half-Life
Infections
Injections, Subcutaneous
Inoculation
Inoculum
Lesions
Major Articles
Male
Medical sciences
Microspheres
Miscellaneous
Placebos
Regression Analysis
Staphylococcal Infections - microbiology
Staphylococcal Infections - prevention & control
Staphylococcus
Staphylococcus aureus - growth & development
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Surgical Wound Infection - microbiology
Surgical Wound Infection - prevention & control
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A model of surgical wound infection that uses low inocula of bacteria and closely simulates clinical infection involved inoculating suspensions of Staphylococcus aureus and dextran microbeads into intermuscular sites on the dorsum of guinea pigs, harvesting lesions at 72–96 h, identifying as a positive end point lesions yielding staphylococci on subculture, and using logistic regression for data analysis. Prophylaxis was placebo, ampicillin, or cefazolin, and three representative strains of S. aureus were used. A highly significant correlation (P < .001) was observed between inoculum sizes and infection rates. Without antimicrobial prophylaxis, ID50 for each strain was
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/166.2.393