Affinity Constants of Naturally Acquired and Vaccine-Induced Anti-Pseudomonas aeruginosa Antibodies in Healthy Adults and Cystic Fibrosis Patients

Naturally acquired anti-Pseudomonas aeruginosa antibody fails to afford protection against repeated P. aeruginosa bronchopulmonary exacerbations in cystic fibrosis (CF) patients. In an effort to explain this phenomenon, the titer and affinity constants of serum anti-lipopolysaccharide (LPS) IgG were...

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Bibliographic Details
Published in:The Journal of infectious diseases 1992-08, Vol.166 (2), p.344-349
Main Authors: Bruderer, Urs, Cryz, Stanley J., Schaad, Urs B., Deusinger, Markus, Que, John U., Lang, Alois B.
Format: Article
Language:English
Subjects:
Antibodies
Antibodies, Bacterial - biosynthesis
Antibodies, Bacterial - blood
Antibody Affinity
Antigens
Bacterial Vaccines - immunology
Bacteriology
Biological and medical sciences
Cystic fibrosis
Cystic Fibrosis - complications
Cystic Fibrosis - immunology
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
Humans
Immune response
Immunization
Immunization, Secondary
Immunoglobulin G - biosynthesis
Infections
Lipopolysaccharides - immunology
Major Articles
Microbiology
Neutrophils
Opsonin Proteins - immunology
Phagocytosis
Pseudomonas aeruginosa
Pseudomonas aeruginosa - immunology
Pseudomonas Infections - prevention & control
Vaccination
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
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Summary:Naturally acquired anti-Pseudomonas aeruginosa antibody fails to afford protection against repeated P. aeruginosa bronchopulmonary exacerbations in cystic fibrosis (CF) patients. In an effort to explain this phenomenon, the titer and affinity constants of serum anti-lipopolysaccharide (LPS) IgG were determined in five study groups: healthy adults before and after immunization with a polyvalent LPS-based vaccine, healthy noncolonized CF patients before and after immunization, nonimmunized CF patients with significantly elevated anti-LPS antibody titers without documented colonization, recently colonized CF patients before and after immunization, and nonimmunized CF patients chronically colonized with P. aeruginosa. Immunization elicited a significant rise in total anti-LPS immunoglobulin levels and affinity constants in both healthy adults and CF patients. Although chronically colonized patients had elevated levels of total anti-LPS antibody, these antibodies possessed affinities at least tOO-fold less than those of vaccine-induced antibodies.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/166.2.344