Production of anthocyanins by Catharanthus roseus

A stable cell suspension line of Catharanthus roseus producing anthocyanin was obtained. In this strain it was found that approximately 30% of cells regularly accumulated these metabolites and that anthocyanin accumulation occurred between the second half of log phase and the stationary phase of the...

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Bibliographic Details
Published in:Fitoterapia 2003-02, Vol.74 (1), p.62-67
Main Authors: Filippini, R, Caniato, R, Piovan, A, Cappelletti, E.M
Format: Article
Language:English
Subjects:
Anthocyanins
Anthocyanins - biosynthesis
Anthocyanins - chemistry
Apocynaceae
Biological and medical sciences
Catharanthus - chemistry
Catharanthus - cytology
Catharanthus - growth & development
Catharanthus - physiology
Catharanthus roseus
Cell Line
Cells, Cultured
Flowers
General pharmacology
Humans
Mass Spectrometry
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Phytotherapy
Plant Extracts - chemistry
Regenerated plant
Regeneration
Suspension cultures
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Summary:A stable cell suspension line of Catharanthus roseus producing anthocyanin was obtained. In this strain it was found that approximately 30% of cells regularly accumulated these metabolites and that anthocyanin accumulation occurred between the second half of log phase and the stationary phase of the culture growth cycle. The anthocyanins in the suspension cultures were compared with those biosynthetized in the flowers both of regenerated by somatic embryogenesis and field-grown plants. Six anthocyanins were identified in all the examined samples, three 3- O-glucosides and three 3- O-(6- O- p-coumaroyl) glucosides of petunidin, malvidin and hirsutidin. The hirsutidin coumaroyl glucoside has not been reported previously, and was predominat in all samples. The anthocyanin relative content was similar for cell suspensions and flowers from regenerated plants but different from field-grown plant flowers; instead, the total content was almost the same for the two flower types and higher compared to suspension culture content.
ISSN:0367-326X
DOI:10.1016/S0367-326X(02)00296-4