Influence of temperature on corneas stored in culture medium. A comparative study using functional and morphological methods

. Purpose:  To investigate the influence of storage temperature on corneal swelling and on endothelial morphology in cultured corneas. Material and methods:  Forty‐eight rabbit corneas were separated into four groups of 12. The corneas were stored in culture medium at 37 ° (group 37), 34 ° (group 34...

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Bibliographic Details
Published in:Acta ophthalmologica Scandinavica 2003-02, Vol.81 (1), p.54-59
Main Authors: Sandboe, Frode D., Medin, Walther, Frøslie, Kathrine Frey
Format: Article
Language:English
Subjects:
Animals
Cell Count
Cornea
Corneal Edema - pathology
Corneal Edema - physiopathology
Cryopreservation
Culture Media
culture medium
Endothelium, Corneal - ultrastructure
Microscopy, Electron, Scanning
Organ Preservation
Organ Size
Rabbits
scanning electron microscopy
swelling
Temperature
weight recordings
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Summary:. Purpose:  To investigate the influence of storage temperature on corneal swelling and on endothelial morphology in cultured corneas. Material and methods:  Forty‐eight rabbit corneas were separated into four groups of 12. The corneas were stored in culture medium at 37 ° (group 37), 34 ° (group 34), 31 ° (group 31) and 23 ° (room temperature) (group 23), respectively. All the corneas were monitored by weight recordings on days 0, 1, 2, 3, 4 and 7. On day 7, corneas were prepared for scanning electron microscopy and endothelial cell counts were performed. Results:  Lowering the temperature of the culture medium resulted in less swelling. Both temperature and storage time had significant effects on corneal swelling (p 
ISSN:1395-3907
1600-0420
DOI:10.1034/j.1600-0420.2003.00002.x