Stimulation of Na Absorption by the Antiasthmatic Kampo Drug Saiboku-To in Cultured Airway Epithelium

To study the effect of the Kampo drug Saiboku-to (TJ-96) on ion transport function of airway epithelial cells, we studied bioelectric properties of cultured tracheal epithelium from dogs under short-circuit conditions in vitro. Addition of TJ-96 (1 mg/ml) to the mucosal solution of the Ussing chambe...

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Bibliographic Details
Published in:Japanese Journal of Pharmacology 1992, Vol.58(1), pp.47-53
Main Authors: Tamaoki, Jun, Takeyama, Kiyoshi, Chiyotani, Atsushi, Sakai, Noritaka, Takizawa, Takao, Konno, Kimio
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cells, Cultured
Cyclic AMP - analysis
Dogs
Drugs, Chinese Herbal - pharmacology
Epithelium - metabolism
Female
Histamine and antagonists. Allergy
Histamine H1 Antagonists - pharmacology
Male
Medical sciences
Medicine, Kampo
Pharmacology. Drug treatments
Potentiometry
Sodium-Potassium-Exchanging ATPase - drug effects
Trachea - metabolism
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Summary:To study the effect of the Kampo drug Saiboku-to (TJ-96) on ion transport function of airway epithelial cells, we studied bioelectric properties of cultured tracheal epithelium from dogs under short-circuit conditions in vitro. Addition of TJ-96 (1 mg/ml) to the mucosal solution of the Ussing chamber increased the epithelial short-circuit current (SCC) from 6.5 ± 0.7 to 11.4 ± 1.6μA/cm2 (P < 0.001). This effect was dose-dependent, with the maximal increase from the baseline value and the concentration required to produce a half-maximal effect (EC50) being 70.5 ± 12.6% (P
ISSN:0021-5198
1347-3506
DOI:10.1254/jjp.58.47