Potassium channel expression level is dependent on the proliferation state in the GH3 pituitary cell line

1  Laboratoire de Physiologie et Physiopathologie de la Signalisation Cellulaire, CNRS UMR 5543, Université de Bordeaux 2, 33076 Cedex Bordeaux; and 2  Laboratoire d'Immunologie et Cytométrie, Institut Bergonié, 33000 Bordeaux, France Previously, we showed that the peak density of the transient...

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Published in:American Journal of Physiology: Cell Physiology 2003-04, Vol.284 (4), p.C1054-C1064
Main Authors: Czarnecki, Antonny, Dufy-Barbe, Luce, Huet, Sylvie, Odessa, Marie-Francoise, Bresson-Bepoldin, Laurence
Format: Article
Language:English
Subjects:
Animals
Cell Cycle
Cell Division - physiology
Cell Line
Electrophysiology
G1 Phase - physiology
Kv1.4 Potassium Channel
Pituitary Gland - cytology
Pituitary Gland - metabolism
Pituitary Gland - physiology
Potassium Channels - metabolism
Potassium Channels - physiology
Potassium Channels, Voltage-Gated
Resting Phase, Cell Cycle - physiology
Shal Potassium Channels
Time Factors
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Summary:1  Laboratoire de Physiologie et Physiopathologie de la Signalisation Cellulaire, CNRS UMR 5543, Université de Bordeaux 2, 33076 Cedex Bordeaux; and 2  Laboratoire d'Immunologie et Cytométrie, Institut Bergonié, 33000 Bordeaux, France Previously, we showed that the peak density of the transient outward K + current ( I to ) expressed in GH3 cells was different in the S phase than in other phases of the cell cycle. Using cell synchronization, we show here that I to drops precisely at the quiescent (G 0 phase)/proliferating transition. This change is not due to a modification in the voltage dependence of I to , but rather to a modification in its inactivation kinetics. Molecular determination of K + channel subunits showed that I to required the expression of Kv1.4, Kv4.1, and Kv4.3. We found that the increase in I to density during the quiescent state was accompanied by an increase in Kv1.4 protein expression, whereas Kv4.3 expression remained unchanged. We further demonstrate that the link between I to expression and cell proliferation is not mediated by variations in cell excitability. These results provide new evidence for the cell cycle dependence of I to expression, which could be relevant in understanding the mechanisms leading to pituitary adenomas. Kv1.4; cell growth; excitable cell; transient outward K + current
ISSN:0363-6143
1522-1563
DOI:10.1152/ajpcell.00446.2002