Loading…
Potassium channel expression level is dependent on the proliferation state in the GH3 pituitary cell line
1 Laboratoire de Physiologie et Physiopathologie de la Signalisation Cellulaire, CNRS UMR 5543, Université de Bordeaux 2, 33076 Cedex Bordeaux; and 2 Laboratoire d'Immunologie et Cytométrie, Institut Bergonié, 33000 Bordeaux, France Previously, we showed that the peak density of the transient...
Saved in:
| Published in: | American Journal of Physiology: Cell Physiology 2003-04, Vol.284 (4), p.C1054-C1064 |
|---|---|
| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c433t-578cdaaa4b056a9d3f1bcf91ab600049d9ad9ca678758d800667fe8a6b5e5d093 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c433t-578cdaaa4b056a9d3f1bcf91ab600049d9ad9ca678758d800667fe8a6b5e5d093 |
| container_end_page | C1064 |
| container_issue | 4 |
| container_start_page | C1054 |
| container_title | American Journal of Physiology: Cell Physiology |
| container_volume | 284 |
| creator | Czarnecki, Antonny Dufy-Barbe, Luce Huet, Sylvie Odessa, Marie-Francoise Bresson-Bepoldin, Laurence |
| description | 1 Laboratoire de Physiologie et Physiopathologie de
la Signalisation Cellulaire, CNRS UMR 5543, Université de
Bordeaux 2, 33076 Cedex Bordeaux; and 2 Laboratoire
d'Immunologie et Cytométrie, Institut Bergonié, 33000 Bordeaux, France
Previously, we showed that the peak
density of the transient outward K + current
( I to ) expressed in GH3 cells was different in
the S phase than in other phases of the cell cycle. Using cell
synchronization, we show here that I to drops
precisely at the quiescent (G 0 phase)/proliferating transition. This change is not due to a modification in the voltage dependence of I to , but rather to a modification
in its inactivation kinetics. Molecular determination of K +
channel subunits showed that I to required the
expression of Kv1.4, Kv4.1, and Kv4.3. We found that the increase in
I to density during the quiescent state was
accompanied by an increase in Kv1.4 protein expression, whereas Kv4.3
expression remained unchanged. We further demonstrate that the link
between I to expression and cell proliferation is
not mediated by variations in cell excitability. These results provide
new evidence for the cell cycle dependence of
I to expression, which could be relevant in
understanding the mechanisms leading to pituitary adenomas.
Kv1.4; cell growth; excitable cell; transient outward
K + current |
| doi_str_mv | 10.1152/ajpcell.00446.2002 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73087189</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73087189</sourcerecordid><originalsourceid>FETCH-LOGICAL-c433t-578cdaaa4b056a9d3f1bcf91ab600049d9ad9ca678758d800667fe8a6b5e5d093</originalsourceid><addsrcrecordid>eNp1kEtLxDAUhYMoOj7-gAvJyl3HPNo0dSeDLxB0oeuQNrc2kmlrk-rMvzd1RnTj6sK53zkcDkKnlMwpzdiFfusrcG5OSJqKOSOE7aBZfLCEZoLvohnhgieCpvwAHXr_RiLIRLGPDigTjMginyH71AXtvR2XuGp024LDsOoHiFLXYgcfUbAeG-ihNdAGHNXQAO6HztkaBh0mzgcdANvN6_aO496G0QY9rPHUEDvbwjHaq7XzcLK9R-jl5vp5cZc8PN7eL64ekirlPCRZLiujtU5LkgldGF7TsqoLqksx9S9MoU1RaZHLPJNGEiJEXoPUoswgM6TgR-h8kxsrvo_gg1paP7XQLXSjVzknMqdyAtkGrIbO-wFq1Q92GTsrStQ0sNoOrL4HVtPA0XS2TR_LJZhfy3bRCFxugMa-Np92ANU36zim617X6mZ07hlW4SeZyVSlakFJlqre1NE8_9_80-aPiX8BizCflA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73087189</pqid></control><display><type>article</type><title>Potassium channel expression level is dependent on the proliferation state in the GH3 pituitary cell line</title><source>American Physiological Society Free</source><creator>Czarnecki, Antonny ; Dufy-Barbe, Luce ; Huet, Sylvie ; Odessa, Marie-Francoise ; Bresson-Bepoldin, Laurence</creator><creatorcontrib>Czarnecki, Antonny ; Dufy-Barbe, Luce ; Huet, Sylvie ; Odessa, Marie-Francoise ; Bresson-Bepoldin, Laurence</creatorcontrib><description>1 Laboratoire de Physiologie et Physiopathologie de
la Signalisation Cellulaire, CNRS UMR 5543, Université de
Bordeaux 2, 33076 Cedex Bordeaux; and 2 Laboratoire
d'Immunologie et Cytométrie, Institut Bergonié, 33000 Bordeaux, France
Previously, we showed that the peak
density of the transient outward K + current
( I to ) expressed in GH3 cells was different in
the S phase than in other phases of the cell cycle. Using cell
synchronization, we show here that I to drops
precisely at the quiescent (G 0 phase)/proliferating transition. This change is not due to a modification in the voltage dependence of I to , but rather to a modification
in its inactivation kinetics. Molecular determination of K +
channel subunits showed that I to required the
expression of Kv1.4, Kv4.1, and Kv4.3. We found that the increase in
I to density during the quiescent state was
accompanied by an increase in Kv1.4 protein expression, whereas Kv4.3
expression remained unchanged. We further demonstrate that the link
between I to expression and cell proliferation is
not mediated by variations in cell excitability. These results provide
new evidence for the cell cycle dependence of
I to expression, which could be relevant in
understanding the mechanisms leading to pituitary adenomas.
Kv1.4; cell growth; excitable cell; transient outward
K + current</description><identifier>ISSN: 0363-6143</identifier><identifier>EISSN: 1522-1563</identifier><identifier>DOI: 10.1152/ajpcell.00446.2002</identifier><identifier>PMID: 12620897</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Cell Cycle ; Cell Division - physiology ; Cell Line ; Electrophysiology ; G1 Phase - physiology ; Kv1.4 Potassium Channel ; Pituitary Gland - cytology ; Pituitary Gland - metabolism ; Pituitary Gland - physiology ; Potassium Channels - metabolism ; Potassium Channels - physiology ; Potassium Channels, Voltage-Gated ; Resting Phase, Cell Cycle - physiology ; Shal Potassium Channels ; Time Factors</subject><ispartof>American Journal of Physiology: Cell Physiology, 2003-04, Vol.284 (4), p.C1054-C1064</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c433t-578cdaaa4b056a9d3f1bcf91ab600049d9ad9ca678758d800667fe8a6b5e5d093</citedby><cites>FETCH-LOGICAL-c433t-578cdaaa4b056a9d3f1bcf91ab600049d9ad9ca678758d800667fe8a6b5e5d093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12620897$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Czarnecki, Antonny</creatorcontrib><creatorcontrib>Dufy-Barbe, Luce</creatorcontrib><creatorcontrib>Huet, Sylvie</creatorcontrib><creatorcontrib>Odessa, Marie-Francoise</creatorcontrib><creatorcontrib>Bresson-Bepoldin, Laurence</creatorcontrib><title>Potassium channel expression level is dependent on the proliferation state in the GH3 pituitary cell line</title><title>American Journal of Physiology: Cell Physiology</title><addtitle>Am J Physiol Cell Physiol</addtitle><description>1 Laboratoire de Physiologie et Physiopathologie de
la Signalisation Cellulaire, CNRS UMR 5543, Université de
Bordeaux 2, 33076 Cedex Bordeaux; and 2 Laboratoire
d'Immunologie et Cytométrie, Institut Bergonié, 33000 Bordeaux, France
Previously, we showed that the peak
density of the transient outward K + current
( I to ) expressed in GH3 cells was different in
the S phase than in other phases of the cell cycle. Using cell
synchronization, we show here that I to drops
precisely at the quiescent (G 0 phase)/proliferating transition. This change is not due to a modification in the voltage dependence of I to , but rather to a modification
in its inactivation kinetics. Molecular determination of K +
channel subunits showed that I to required the
expression of Kv1.4, Kv4.1, and Kv4.3. We found that the increase in
I to density during the quiescent state was
accompanied by an increase in Kv1.4 protein expression, whereas Kv4.3
expression remained unchanged. We further demonstrate that the link
between I to expression and cell proliferation is
not mediated by variations in cell excitability. These results provide
new evidence for the cell cycle dependence of
I to expression, which could be relevant in
understanding the mechanisms leading to pituitary adenomas.
Kv1.4; cell growth; excitable cell; transient outward
K + current</description><subject>Animals</subject><subject>Cell Cycle</subject><subject>Cell Division - physiology</subject><subject>Cell Line</subject><subject>Electrophysiology</subject><subject>G1 Phase - physiology</subject><subject>Kv1.4 Potassium Channel</subject><subject>Pituitary Gland - cytology</subject><subject>Pituitary Gland - metabolism</subject><subject>Pituitary Gland - physiology</subject><subject>Potassium Channels - metabolism</subject><subject>Potassium Channels - physiology</subject><subject>Potassium Channels, Voltage-Gated</subject><subject>Resting Phase, Cell Cycle - physiology</subject><subject>Shal Potassium Channels</subject><subject>Time Factors</subject><issn>0363-6143</issn><issn>1522-1563</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNp1kEtLxDAUhYMoOj7-gAvJyl3HPNo0dSeDLxB0oeuQNrc2kmlrk-rMvzd1RnTj6sK53zkcDkKnlMwpzdiFfusrcG5OSJqKOSOE7aBZfLCEZoLvohnhgieCpvwAHXr_RiLIRLGPDigTjMginyH71AXtvR2XuGp024LDsOoHiFLXYgcfUbAeG-ihNdAGHNXQAO6HztkaBh0mzgcdANvN6_aO496G0QY9rPHUEDvbwjHaq7XzcLK9R-jl5vp5cZc8PN7eL64ekirlPCRZLiujtU5LkgldGF7TsqoLqksx9S9MoU1RaZHLPJNGEiJEXoPUoswgM6TgR-h8kxsrvo_gg1paP7XQLXSjVzknMqdyAtkGrIbO-wFq1Q92GTsrStQ0sNoOrL4HVtPA0XS2TR_LJZhfy3bRCFxugMa-Np92ANU36zim617X6mZ07hlW4SeZyVSlakFJlqre1NE8_9_80-aPiX8BizCflA</recordid><startdate>20030401</startdate><enddate>20030401</enddate><creator>Czarnecki, Antonny</creator><creator>Dufy-Barbe, Luce</creator><creator>Huet, Sylvie</creator><creator>Odessa, Marie-Francoise</creator><creator>Bresson-Bepoldin, Laurence</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030401</creationdate><title>Potassium channel expression level is dependent on the proliferation state in the GH3 pituitary cell line</title><author>Czarnecki, Antonny ; Dufy-Barbe, Luce ; Huet, Sylvie ; Odessa, Marie-Francoise ; Bresson-Bepoldin, Laurence</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c433t-578cdaaa4b056a9d3f1bcf91ab600049d9ad9ca678758d800667fe8a6b5e5d093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Cell Cycle</topic><topic>Cell Division - physiology</topic><topic>Cell Line</topic><topic>Electrophysiology</topic><topic>G1 Phase - physiology</topic><topic>Kv1.4 Potassium Channel</topic><topic>Pituitary Gland - cytology</topic><topic>Pituitary Gland - metabolism</topic><topic>Pituitary Gland - physiology</topic><topic>Potassium Channels - metabolism</topic><topic>Potassium Channels - physiology</topic><topic>Potassium Channels, Voltage-Gated</topic><topic>Resting Phase, Cell Cycle - physiology</topic><topic>Shal Potassium Channels</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Czarnecki, Antonny</creatorcontrib><creatorcontrib>Dufy-Barbe, Luce</creatorcontrib><creatorcontrib>Huet, Sylvie</creatorcontrib><creatorcontrib>Odessa, Marie-Francoise</creatorcontrib><creatorcontrib>Bresson-Bepoldin, Laurence</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American Journal of Physiology: Cell Physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Czarnecki, Antonny</au><au>Dufy-Barbe, Luce</au><au>Huet, Sylvie</au><au>Odessa, Marie-Francoise</au><au>Bresson-Bepoldin, Laurence</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Potassium channel expression level is dependent on the proliferation state in the GH3 pituitary cell line</atitle><jtitle>American Journal of Physiology: Cell Physiology</jtitle><addtitle>Am J Physiol Cell Physiol</addtitle><date>2003-04-01</date><risdate>2003</risdate><volume>284</volume><issue>4</issue><spage>C1054</spage><epage>C1064</epage><pages>C1054-C1064</pages><issn>0363-6143</issn><eissn>1522-1563</eissn><abstract>1 Laboratoire de Physiologie et Physiopathologie de
la Signalisation Cellulaire, CNRS UMR 5543, Université de
Bordeaux 2, 33076 Cedex Bordeaux; and 2 Laboratoire
d'Immunologie et Cytométrie, Institut Bergonié, 33000 Bordeaux, France
Previously, we showed that the peak
density of the transient outward K + current
( I to ) expressed in GH3 cells was different in
the S phase than in other phases of the cell cycle. Using cell
synchronization, we show here that I to drops
precisely at the quiescent (G 0 phase)/proliferating transition. This change is not due to a modification in the voltage dependence of I to , but rather to a modification
in its inactivation kinetics. Molecular determination of K +
channel subunits showed that I to required the
expression of Kv1.4, Kv4.1, and Kv4.3. We found that the increase in
I to density during the quiescent state was
accompanied by an increase in Kv1.4 protein expression, whereas Kv4.3
expression remained unchanged. We further demonstrate that the link
between I to expression and cell proliferation is
not mediated by variations in cell excitability. These results provide
new evidence for the cell cycle dependence of
I to expression, which could be relevant in
understanding the mechanisms leading to pituitary adenomas.
Kv1.4; cell growth; excitable cell; transient outward
K + current</abstract><cop>United States</cop><pmid>12620897</pmid><doi>10.1152/ajpcell.00446.2002</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0363-6143 |
| ispartof | American Journal of Physiology: Cell Physiology, 2003-04, Vol.284 (4), p.C1054-C1064 |
| issn | 0363-6143 1522-1563 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73087189 |
| source | American Physiological Society Free |
| subjects | Animals Cell Cycle Cell Division - physiology Cell Line Electrophysiology G1 Phase - physiology Kv1.4 Potassium Channel Pituitary Gland - cytology Pituitary Gland - metabolism Pituitary Gland - physiology Potassium Channels - metabolism Potassium Channels - physiology Potassium Channels, Voltage-Gated Resting Phase, Cell Cycle - physiology Shal Potassium Channels Time Factors |
| title | Potassium channel expression level is dependent on the proliferation state in the GH3 pituitary cell line |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T02%3A47%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Potassium%20channel%20expression%20level%20is%20dependent%20on%20the%20proliferation%20state%20in%20the%20GH3%20pituitary%20cell%20line&rft.jtitle=American%20Journal%20of%20Physiology:%20Cell%20Physiology&rft.au=Czarnecki,%20Antonny&rft.date=2003-04-01&rft.volume=284&rft.issue=4&rft.spage=C1054&rft.epage=C1064&rft.pages=C1054-C1064&rft.issn=0363-6143&rft.eissn=1522-1563&rft_id=info:doi/10.1152/ajpcell.00446.2002&rft_dat=%3Cproquest_cross%3E73087189%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c433t-578cdaaa4b056a9d3f1bcf91ab600049d9ad9ca678758d800667fe8a6b5e5d093%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=73087189&rft_id=info:pmid/12620897&rfr_iscdi=true |