A Cancer-prone Case with a Background of Methylation of p16 Tumor Suppressor Gene

Purpose and Experimental Design: To date, the presence of p16 gene promoter methylation associated with loss of protein expression has been demonstrated frequently in digestive tract cancers. In this study, we tested for the methylation status of p16 promoter in normal tissue specimens using the met...

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Bibliographic Details
Published in:Clinical cancer research 2003-03, Vol.9 (3), p.1053-1056
Main Authors: HIBI, Kenji, KOIKE, Masahiko, NAKAYAMA, Hiroshi, FUJITAKE, Shinichi, KASAI, Yasushi, ITO, Katsuki, AKIYAMA, Seiji, NAKAO, Akimasa
Format: Article
Language:English
Subjects:
Biological and medical sciences
Colorectal Neoplasms - genetics
DNA Methylation
Esophageal Neoplasms - genetics
Esophagus
Gastroenterology. Liver. Pancreas. Abdomen
Genes, p16
Humans
Immunohistochemistry
Lymphatic Metastasis
Male
Medical sciences
Neoplasm Metastasis
Neoplasms - genetics
Promoter Regions, Genetic
Reverse Transcriptase Polymerase Chain Reaction
Stomach Neoplasms - genetics
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Sulfites - pharmacology
Tumors
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Summary:Purpose and Experimental Design: To date, the presence of p16 gene promoter methylation associated with loss of protein expression has been demonstrated frequently in digestive tract cancers. In this study, we tested for the methylation status of p16 promoter in normal tissue specimens using the methylation-specific PCR technique to examine whether p16 methylation already existed in the background of tumors. Results: Aberrant promoter methylation of p16 gene was detected in 1 of 40 esophageal and 1 of 69 gastric and no colorectal epithelium specimens, and these 2 specimens were derived from the same patient. We also found the same methylation change in both tumor and blood cell DNA. Conclusion: These results suggested that the p16 gene was inactivated by methylation in normal background cells of this patient and that other additional factors may promote tumor development in his esophageal and gastric tissues.
ISSN:1078-0432
1557-3265