CAL72: a human osteosarcoma cell line with unique effects on hematopoietic cells

• Published in: European journal of haematology 2003-01, Vol.70 (1), p.43-52
• Main Authors: Rochet, Nathalie, Leroy, Pascale, Far, Dariush Farahi, Ollier, Laurence, Loubat, Agnès, Rossi, Bernard
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Bone Marrow Cells - cytology; Bone Marrow Cells - physiology; Bone Marrow Neoplasms - pathology; CAL72 cell line; CD34+ cells; Cell Communication; Cell Differentiation; Cell Division; chemokines; coculture; Coculture Techniques; cytokines; Diseases of the osteoarticular system; Fetal Blood - cytology; Hematopoiesis; Hematopoietic Stem Cells - cytology; HL-60; HL-60 Cells; Humans; Medical sciences; Osteoblasts - physiology; osteosarcoma; Osteosarcoma - pathology; Tumor Cells, Cultured - cytology; Tumors of striated muscle and skeleton
• ISSN: 0902-4441; 1600-0609
• DOI: 10.1034/j.1600-0609.2003.02766.x

: Permanent osteoblastic cell lines are potential tools to study the interactions between osteoblastic and hematopoietic cells in the bone marrow cavity. In a recent work we have shown that the osteosarcoma cell line CAL72 may be more closely related to normal osteoblasts than the osteosarcoma cells previously described. In the present work we continued the characterisation of the CAL72 cell line with regard to its effects on various hematopoietic cells, in coculture experiments. We show here that CAL72 cells, in contrast to MG‐63 or SaOS‐2 osteosarcoma cell lines, do not inhibit hematopoietic colony formation and sustain the limited expansion of hematopoietic progenitors in a similar way to that described for normal osteoblasts. We also demonstrate that CAL72 cells induce the monocytic differentiation of the promyelocytic HL‐60 cell line like MG‐63 and SaOS‐2, but support a better maturation and a longer survival of the differentiated cells than the two other osteosarcoma cell lines. In order to better understand the differential effects observed between CAL72 and MG‐63 or SaOS‐2, we analysed the cytokine and chemokine mRNA expression of these cells using the RNase protection quantitative assay. We show here that the expression profile of CAL72 is clearly different from that of MG‐63 or SaOS‐2 and may explain, at least in part, its specific effects on hematopoietic cells. Taken together these experiments confirm that CAL72 has particular properties and is an interesting tool to study the role of osteoblastic cells in hematopoietic cell growth and differentiation.