Identification of cDNAs from Japanese pufferfish (Fugu rubripes) and Atlantic salmon (Salmo salar) coding for homologues to tetrapod prion proteins

We identified cDNAs coding for homologues to tetrapod prion proteins (PrPs) in Atlantic salmon (Salmo salar) and Japanese pufferfish (Fugu rubripes), which were termed ‘similar to PrPs’ (stPrPs). Besides significant sequence homologies the fish stPrPs display characteristic structural features in co...

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Bibliographic Details
Published in:FEBS letters 2003-03, Vol.538 (1-3), p.96-100
Main Authors: Oidtmann, Birgit, Simon, Dietrich, Holtkamp, Nikola, Hoffmann, Rudolf, Baier, Michael
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Atlantic salmon
Base Sequence
Blotting, Northern
DNA Primers
DNA, Complementary
doppel protein
Fugu rubripes
Marine
Molecular Sequence Data
Polymerase Chain Reaction
Prion
Prions - chemistry
Prions - genetics
PrP gene
Salmo salar
Salmon
Sequence Homology, Amino Acid
Takifugu
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Summary:We identified cDNAs coding for homologues to tetrapod prion proteins (PrPs) in Atlantic salmon (Salmo salar) and Japanese pufferfish (Fugu rubripes), which were termed ‘similar to PrPs’ (stPrPs). Besides significant sequence homologies the fish stPrPs display characteristic structural features in common with tetrapod PrPs. In addition, two stPrPs were shown to be highly expressed in brain tissue. None of the so far identified PrP-homologues of fish resembles doppel. Hence, the duplication of the PrP gene, which generated doppel, may have occurred not in fish but later in the tetrapod lineage. The identification of fish PrPs provides a basis to address concerns about a possible susceptibility of fish to prion infections.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(03)00149-2