Localization of group-specific epitopes on the major capsid protein of group A rotavirus

• Published in: Journal of general virology 1992-04, Vol.73 (4), p.907-914
• Main Authors: Kohli, E, Maurice, L, Vautherot, J. F, Bourgeois, C, Bour, J. B, Cohen, J, Pothier, P
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Antibodies, Monoclonal; Antigens, Viral; Binding, Competitive; Biological and medical sciences; Capsid - classification; Capsid - immunology; Capsid Proteins; Cattle - microbiology; Epitopes - classification; Epitopes - immunology; Fundamental and applied biological sciences. Psychology; Microbiology; Molecular Sequence Data; Morphology, structure, chemical composition, physicochemical properties; Peptide Fragments - immunology; Peptides - immunology; Rotavirus - classification; Rotavirus - immunology; Virology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-73-4-907

1 Laboratory of Virology, Facultés de Médecine et de Pharmacie, Boulevard Jeanne d'Arc, 21033 Dijon Cedex and 2 Laboratoire de Virologie et d'Immunologie Moléculaires, INRA, C.R.J. Domaine de Vilvert, 78350 Jouy-en-Josas, France Chemical cleavage of the VP6 protein of bovine rotavirus showed that VP6-specific monoclonal antibodies (MAbs) reacted with the amino acid sequence between glycine 48 and asparagine 107. Furthermore, three synthetic peptides (amino acids 48 to 64, 60 to 75 and 91 to 108) containing part of this sequence and 22 consecutive overlapping heptapeptides corresponding to the region between amino acids 48 and 75 were analysed for their immunoreactivity using group-specific MAbs. The MAbs recognized peptides 48–64 and/or 60–75, and a set of overlapping heptapeptides located between residues 53 (asparagine) and 67 (glycine), which have two short sequences in common: IRNW (residues 56 to 59), recognized by MAb RV-133, and (NW)NFD (residues 58/60 to 62), recognized by MAbs RV-50, -1026 and -443. These results indicate that the sequence between amino acid residues 48 and 75 is present in one of the immuno-dominant sites of VP6. Received 16 October 1991; accepted 31 December 1991.