Hypovirulence of chestnut blight fungus conferred by an infectious viral cDNA

Strains of the chestnut blight fungus Cryphonectria parasitica that contain viral double-stranded RNAs often exhibit reduced virulence. Such hypovirulent strains act as biocontrol agents by virtue of their ability to convert virulent strains to hypovirulence after anastomosis. Transformation of viru...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1992-08, Vol.257 (5071), p.800-803
Main Authors: Choi, G.H. (Roche Research Center, Nutley, NJ), Nuss, D.L
Format: Article
Language:English
Subjects:
ADN
Antisense DNA
ARN
Biological and medical sciences
Biotechnological microorganisms
Biotechnology
CASTANEA SATIVA
Complementary DNA
Control
CONTROL BIOLOGICO
CRYPHONECTRIA PARASITICA
DNA, Viral - genetics
Double stranded RNA
Fundamental and applied biological sciences. Psychology
Fungal diseases of plants
Fungi
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
LUTTE BIOLOGIQUE
Microbial biotechnology
Microbiology
Multidisciplinary Sciences
Mycology
Open Reading Frames
Parasitism
Phenotype
Phenotypes
Phenotypic traits
Plant Diseases - microbiology
Plant fungal diseases
Plasmids
PODER PATOGENO
POUVOIR PATHOGENE
Restriction Mapping
RNA
RNA, Double-Stranded - genetics
RNA, Viral - genetics
Science & Technology
Science & Technology - Other Topics
Transformation, Genetic
Virulence
Virulence (Microbiology)
Virulence - genetics
VIRUS DE LAS PLANTAS
VIRUS DES VEGETAUX
Xylariales - genetics
Xylariales - pathogenicity
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Description
Summary:Strains of the chestnut blight fungus Cryphonectria parasitica that contain viral double-stranded RNAs often exhibit reduced virulence. Such hypovirulent strains act as biocontrol agents by virtue of their ability to convert virulent strains to hypovirulence after anastomosis. Transformation of virulent C. parasitica strains with a full-length complementary DNA copy of a hypovirulence-associated viral RNA conferred the complete hypovirulence phenotype. Cytoplasmic double-stranded RNA was resurrected from the chromosomally integrated complementary DNA copy and was able to convert compatible virulent strains to hypovirulence. These results establish viral double-stranded RNA as the causal agent of hypovirulence and demonstrate the feasibility of engineering hypovirulent fungal strains
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1496400