A tripartite microbial reporter gene system for real-time assays of soil nutrient status

Plant-derived carbon is the substrate which drives the rate of microbial assimilation and turnover of nutrients, in particular N and P, within the rhizosphere. To develop a better understanding of rhizosphere dynamics, a tripartite reporter gene system has been developed. We used three lux-marked Ps...

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Bibliographic Details
Published in:FEMS microbiology letters 2003-03, Vol.220 (1), p.35-39
Main Authors: Standing, Dominic, Meharg, Andy A, Killham, Ken
Format: Article
Language:English
Subjects:
Acyltransferases
Bacterial Proteins
Bio-available nutrients
Biological and medical sciences
bioluminescence
carbon
Carbon - metabolism
Computer Systems
Fundamental and applied biological sciences. Psychology
Genes, Reporter
Glucose - metabolism
Glucose - pharmacology
in vivo studies
Luminescent Measurements
Microbiology
nitrates
Nitrates - metabolism
Nitrates - pharmacology
Nitrogen - metabolism
nutrients
Oxidoreductases
P. fluorescens DF57 P9
Phosphates - metabolism
Phosphates - pharmacology
Phosphorus - metabolism
Pseudomonas fluorescens
Pseudomonas fluorescens - genetics
Pseudomonas fluorescens - growth & development
Pseudomonas fluorescens - metabolism
Pseudomonas fluorescens DF57 N3
Recombinant Fusion Proteins - analysis
Reporter gene
reporter genes
rhizosphere
Soil - analysis
Soil Microbiology
soil nutrients
soil water
Vibrio - genetics
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Summary:Plant-derived carbon is the substrate which drives the rate of microbial assimilation and turnover of nutrients, in particular N and P, within the rhizosphere. To develop a better understanding of rhizosphere dynamics, a tripartite reporter gene system has been developed. We used three lux-marked Pseudomonas fluorescens strains to report on soil (1) assimilable carbon, (2) N-status, and (3) P-status. In vivo studies using soil water, spiked with C, N and P to simulate rhizosphere conditions, showed that the tripartite reporter system can provide real-time assessment of carbon and nutrient status. Good quantitative agreement for bioluminescence output between reference material and soil water samples was found for the C and P reporters. With regard to soil nitrate, the minimum bioavailable concentration was found to be greater than that analytically detectable in soil water. This is the first time that bioavailable soil C, N and P have been quantified using a tripartite reporter gene system.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(03)00057-0