Interleukin 1beta and tumour necrosis factor alpha secreting cells are increased in the peripheral blood of patients with primary Sjögren's syndrome

To study systemic alterations of cytokine secreting peripheral blood mononuclear cells (PBMC) in primary Sjögren's syndrome (pSS) and their relation to common clinical and immunological manifestations of this disease. PBMC spontaneously secreting tumour necrosis factor alpha (TNFalpha), interle...

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Published in:Annals of the rheumatic diseases 2003-04, Vol.62 (4), p.359-361
Main Authors: Willeke, P, Schotte, H, Schlüter, B, Erren, M, Becker, H, Dyong, A, Mickholz, E, Domschke, W, Gaubitz, M
Format: Article
Language:English
Subjects:
Adult
Aged
Cells, Cultured
Enzyme-Linked Immunosorbent Assay - methods
Female
Humans
Interleukin-1 - blood
Leukocytes, Mononuclear - immunology
Male
Middle Aged
Sjogren's Syndrome - blood
Sjogren's Syndrome - immunology
Tumor Necrosis Factor-alpha - metabolism
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container_issue 4
container_start_page 359
container_title Annals of the rheumatic diseases
container_volume 62
creator Willeke, P
Schotte, H
Schlüter, B
Erren, M
Becker, H
Dyong, A
Mickholz, E
Domschke, W
Gaubitz, M
description To study systemic alterations of cytokine secreting peripheral blood mononuclear cells (PBMC) in primary Sjögren's syndrome (pSS) and their relation to common clinical and immunological manifestations of this disease. PBMC spontaneously secreting tumour necrosis factor alpha (TNFalpha), interleukin 1beta (IL1beta), and interleukin 6 (IL6) were assessed by enzyme linked immunospot (ELISPOT) analysis in a cohort of 31 patients with pSS fulfilling the modified European classification criteria. Nineteen healthy volunteers served as controls. ELISPOT results were correlated with glandular and extraglandular manifestations and autoantibody titres-that is, rheumatoid factor (RF) isotypes, anti-Ro/SS-A, anti-La/SS-B as determined by an enzyme linked immunosorbent assay (ELISA) technique. The number of TNFalpha and IL1beta secreting cells was significantly higher in patients with pSS than in controls. No differences were detected in the number of IL6 secreting PBMC. Patients with recurrent parotid swelling (RPS) had a significantly increased number of IL1beta secreting PBMC. Moreover, the number of IL1beta secreting PBMC correlated with the disease duration (r(s)=0.479; p
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subjects Adult
Aged
Cells, Cultured
Enzyme-Linked Immunosorbent Assay - methods
Female
Humans
Interleukin-1 - blood
Leukocytes, Mononuclear - immunology
Male
Middle Aged
Sjogren's Syndrome - blood
Sjogren's Syndrome - immunology
Tumor Necrosis Factor-alpha - metabolism
title Interleukin 1beta and tumour necrosis factor alpha secreting cells are increased in the peripheral blood of patients with primary Sjögren's syndrome
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