Fold prediction and comparative modeling of Bdm1: a probable alpha/beta hydrolase associated with hot water epilepsy

Hot water epilepsy (HWE) is a benign and rare form of reflex epilepsy that occurs most commonly in humans. Bdm1 is one of the proteins whose mRNA transcript is overexpressed during HWE in a rat model. We show, by sequence analysis and fold recognition methods, that Bdm1 has strong structural similar...

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Bibliographic Details
Published in:Journal of molecular modeling 2003-02, Vol.9 (1), p.3-8
Main Authors: Bhaduri, Anirban, Krishnaswamy, Lakshminarasimhan, Ullal, Gautam R, Panicker, Mitradas M, Sowdhamini, Ramanathan
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Binding Sites
Catalytic Domain - genetics
Epilepsy, Reflex - enzymology
Epilepsy, Reflex - genetics
Humans
Hydrolases - chemistry
Hydrolases - genetics
Hydrolases - metabolism
Hydrolysis
Models, Molecular
Molecular Sequence Data
Muscle Proteins - chemistry
Muscle Proteins - genetics
Muscle Proteins - metabolism
Nerve Tissue Proteins - chemistry
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Protein Conformation
Protein Folding
Rats
Sequence Alignment
Sequence Homology, Amino Acid
Substrate Specificity
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Summary:Hot water epilepsy (HWE) is a benign and rare form of reflex epilepsy that occurs most commonly in humans. Bdm1 is one of the proteins whose mRNA transcript is overexpressed during HWE in a rat model. We show, by sequence analysis and fold recognition methods, that Bdm1 has strong structural similarities to alpha/beta hydrolases like the thioesterases. A three-dimensional model derived by comparative modeling methods allowed the search for catalytic residues using a flexible functional template characteristic of these enzymes. We predict that Bdm1 might be regulated by homocysteine levels by means of direct participation in degradation pathways.
ISSN:1610-2940
0948-5023
DOI:10.1007/s00894-002-0102-0